Cultivation-independent analysis of 16S rRNA gene sequences in vaginal samples revealed two previously unrecognized, uncultivated Megasphaera-like phylotypes. Phylogenetic analysis and environmental distribution suggest that these Megasphaera types may be unique to the vaginal environment. Quantitative PCR suggests that both phylotypes are present in higher concentrations in women with bacteria...

Combined Approaches for Characterization of an Uncultivated Magnetotactic coccus from Lake Miyun near Beijing Yongxin Pan a; Wei Lin a; Lanxiang Tian a; Rixiang Zhu a; Nikolai Petersen b a Bio-geomagnetism Group, Paleomagnetism and Geochronology Laboratory (SKL-LE), Institute of Geology and Geophysics, Chinese Academy of Sciences, Beijing, China b Department of Earth and Environmental Science, ...

Intra-amniotic infection and inflammation are major causes of preterm birth (PTB). However, intra-amniotic inflammation is often detected in the absence of infection. This may partly be due to the culturing methods employed in hospital laboratories, which are unable to detect the uncultivated species. In this study, intra-amniotic microbial infections associated with PTB were examined by both c...

Permanent grasslands provide a healthy and undisturbed environment. We investigated how mulching altered the soil physicochemical parameters, earthworm abundance, biomass, species composition vegetation cover compared to grassland cultivated alfalfa field. Microplots (2 × 2 m) were mulched with either weed control fabric (WF) alone or WF combined straw (WF + S) on grow tomato in ?sagárd (Nógrád...

Microbes have conventionally been studied in pure culture obtained on culture media in the laboratory. With very limited media options at the disposal of a microbiologist as compared to the vast diversity of microbes that are known to exist, it is not surprising that only about 1-10% of them have been cultivated so far [1]. However, since microbes are the key components of every process on the ...

This paper is dealing with soil erosion assessment using two different approaches: nuclear model and potential method, also known as Gavrilovic's method. Complex valley systems on Titel Loess Plateau were selected for investigation. Radiocaesium favored in many studies an optimal tracer due to its relatively long half-life, negligible renewal the environment strongly binding ability onto partic...

The draft genome of an uncultivated bacterium affiliated with the Peptococcaceae was reconstructed by co-assembling Illumina MiSeq sequences from three single cells sorted by microfluidics from two methanogenic alkane-degrading cultures. Peptococcaceae SCADC (short-chain alkane-degrading culture) may be genetically capable of anaerobic alkane activation by fumarate addition in the absence of su...