Electrical uncoupling of cardiac myocytes during ischemia is delayed by ischemic preconditioning. This presumably adaptive response may limit development of arrhythmia substrates. To elucidate responsible mechanisms, we studied isolated, perfused rat hearts subjected to a standard preconditioning protocol of 3 cycles of 3 minutes of global no-flow ischemia each followed by 5 minutes of reperfus...

introduction: reverse transcriptase-polymerase chain reaction (rt-pcr) assay is a useful tool for the detection of fusion transcript resulting from specific chromosomal translocation of the leukemia cells. a specific chromosomal abnormality, the philadelphia chromosome (ph), is present in 90% to 95% of cml patients.the aberration results from a reciprocal translocation between chromosome 9 and ...

Just as total body energy expenditure in animals can be classified into that which supports resting energy metabolism, work, growth, etc., cellular energy expenditure can similarly be classified. Our overall objective is to examine the metabolic origins of cellular energy expenditure, differentiating between metabolic states where cells are at relative rest, and where cellular energy expenditur...

Rieske non-heme iron oxygenases (ROs) are primarily known for their ability to catalyze the stereoselective formation of vicinal cis-diols in a single step, endowing valuable products pharmaceutical and chemical applications. In addition, ROs can several other oxidation reactions with high regio- stereoselectivity typically broad substrate scope. Owing dependence on multicomponent electron tran...

At the end of the 20th cen tury mo lec u lar ge net ics has been the cen tral theme in bi o log i cal thought. No body ques tions the im por tance of ge net ics, nor ar gues that DNA plays the role of the blue print of life for all com po nents of the liv ing cell. How ever, we still do not un der stand the gen eral mech a nism of the cell func tion and de vel op ment. The se quence of the hu m...

The studies summarized here clearly show that the phosphorylation of one or more serine residues (S635, S639, S649 and/or S652) present in the C-terminal tail of the LHR is necessary, but not sufficient, for the agonist-induced uncoupling of the LHR from adenylate cyclase and for the endocytosis of the agonist-receptor complex. Simultaneous mutation of these four serines to alanines decreases t...

In adult man, brown fat can be detected in perinephric fat depots by visual inspection, electron microscopy and nucleotide binding to the tissue-specific uncoupling protein. The 32 kDa uncoupling protein is functionally active, showing a nucleotide-sensitive conductance to protons and an uncoupling response to fatty acids. The amount of uncoupling protein in human mitochondria is equivalent to ...

Uncoupling mitochondrial proteins (UCPs) belong to a discrete family within the mitochondrial anion carrier superfamily. Several uncoupling protein types have been found in mitochondria from mammals and plants, as well as in fishes, fungi, and protozoa. Mammalian UCPs and plant uncoupling proteins (PUMPs) form five distinct subfamilies. Only subfamily III contains both plant and animal uncoupli...

Eo si nop hil ca tio nic pro tein (ECP) is a he te ro ge neous mo le cu le ori gi na ti ng from ac ti va ted eo si nop hil gra nu lo cytes. Bio lo gi cal ac ti vi ty and the cel lu lar con te nt of ECP are de ter mi ned by ge ne tic and pos ttran sla tio nal fac to rs. Se ve ral sin gle nuc leo ti de po lymor phis ms (SNPs) in hu man ECP ge ne (RNASE3) ha ve been des cri bed so far. ECP is a me...

We provide evidence that ablation or inhibition of, uncoupling protein 1 increases the rate of reactive oxygen containing species production by mitochondria from brown adipose tissue, no matter what electron transport chain substrate is used (succinate, glycerol-3-phosphate or pyruvate/malate). Consistent with these data are our observations that (a) the mitochondrial membrane potential is maxi...