The active-site cleft of bovine pancreatic ribonuclease A (RNase A) is lined with cationic residues that interact with a bound nucleic acid. Those residues interacting with the phosphoryl groups comprise the P0, P1, and P2 subsites, with the scissile P-O5' bond residing in the P1 subsite. Coulombic interactions between the P0 and P2 subsites and phosphoryl groups of the substrate were character...

[reaction: see text] A reliable and efficient synthetic route to UDP-alpha-D-galactofuranose (UDP-Galf) has been developed. Reaction of UMP-N-methylimidazolide with Galf 1-phosphate proceeds rapidly to provide UDP-Galf with excellent reproducibility and in a yield approximately twice as high as those reported previously.

Complex U consists of orotate phosphoribosyltransferase (EC 2.4.2.10) and orotidineG’-phosphate decarboxylase (EC 4.1.1.23), the last two enzymes of the de nova pathway for UMP biosynthesis. Earlier studies had shown that orotate and magnesium 5-phosphoribosyl-1-pyrophosphate, substrates for the transferase, and orotidine 5’-monophosphate (OMP), the product of the transferase and also the subst...

5'-Ribonucleotides such as sodium 5'-inosinate (5'-IMP) , sodium 5'-guanylate (5'-GMP) and sodium 5'-xanthylate possess strong flavor-enhancing ability for humans when they are added in a minute quantity to monosodium glutamate (MSG), but little enhancement of taste occur by the addition of sodium 5'-uridylate (5'-UMP) and 5'-cytidylate (5'-CMP) to MSG5). ADACHI1) showed enhancement of the resp...

Galactose-1-phosphate uridylyltransferase catalyzes the reaction of UDP-glucose with galactose 1-phosphate to form UDP-galactose and glucose 1-phosphate during normal cellular metabolism. The reaction proceeds through a double displacement mechanism characterized by the formation of a stable nucleotidylated histidine intermediate. This paper describes the preparation of the uridylyl-enzyme comp...

We have suggested an important role of the pyrH gene during the infectious process of Vibrio vulnificus. Previously, we have identified 12 genes expressed preferentially during human infections by using in vivo-induced antigen technology. Among the in vivo-expressed genes, pyrH encodes UMP kinase catalyzing UMP phosphorylation. Introduction of a deletion mutation to the pyrH gene was lethal to ...

Inter-turn short circuit of field windings (ISCFW) may cause the field current of a generator to increase, output reactive power to decrease, and unit vibration to intensify, seriously affecting its safe and stable operation. Full integration of mechanical and electrical characteristics can improve the sensitivity of online monitoring, and detect the early embryonic period fault of small turns....

Abstract This study investigated the reaction of German stock market volatility (Dax index) to European Central Bank (ECB)’s unconventional monetary policy (UMP) announcements. The financial crisis 2008 proved that traditional policy’s tool (the short -term interest rate) has lost its effectiveness meet new challenges. So, key central banks, ECB included, had implement new, untested and nonstan...

Getting a decent job and livelihood is basic right of every citizen as that must be obtained. In its implementation, it cannot denied there are problems in employment, one which concerns wages. However, principle employers prohibited from paying wages to workers/employees lower than the minimum wage. The purpose analyzing implementation provision wage expressly regulated Article 23 paragraph (3...