H5N1 avian influenza viruses demonstrate different phenotypes, such as pathogenicity after one or serial passages in mammalian hosts or cells. To establish the molecular basis of these phenotypes, we cloned isolates from the lungs of mice infected with human A/Vietnam/1194/2004 (H5N1) influenza virus. Large-plaque isolates were less pathogenic to mice than small-plaque isolates. Genome sequenci...

Uji karakteristik pektin kulit buah sukun (Artocarpus altilis (Park.) Fosberg) dan uji kemampuan adsorpsi logam berat pada limbah laboratorium stifera Semarang. Tujuan penelitian ini yaitu menentukan kondisi optimum dalam menyerap ion Pb2+ Cd2+ terhadap parameter variasi berat, lama waktu kontak, pH ukuran partikel serta persentase penurunan kadar cair farmasi. Metode isolasi dilakukan dengan m...

a batch adsorption study was applied to investigate the adsorption of pb2+ ions from aqueous solutionby nanographene (ng).the adsorption percentage of pb2+ions onto ng samples at different initial ph,contact time (tc), adsorbent dosage (mng) and temperature (t) were investigated. the results showedthat ng is an excellent adsorbent for pb2+ions removal from aqueous solutions with an adsorptionpe...

Environmental and occupational exposure to lead still generates concern, and recent studies have focused such concern on the role of body burden of lead during the fetal/neonatal period, especially in the genesis of disturbed central nervous system development. This discussion provides some comparative observations on the neurotoxicity of inorganic and organic lead species. The characteristics ...

The aim of this study was to determine the in vitro inhibitory effects some metal ions (silver ion (Ag+), cadmium (Cd2+), cobalt (Co2+), copper (Cu2+), nickel (Ni2+), lead (Pb2+) and zinc (Zn2+)) on glutathione reductase (GR) enzyme activities that purified from gill, kidney liver tissues Capoeta umbla. For purpose, C. umbla freshwater fish using ammonium sulfate precipitation affinity column c...

Avian influenza viruses pose a serious pandemic threat to humans. Better knowledge on cross-species adaptation is important. This study examined the replication and transcription efficiency of ribonucleoprotein complexes reconstituted by plasmid co-transfection between H5N1, H1N1pdm09 and H3N2 influenza A viruses, and to identify mutations in the RNA polymerase subunit that affect human adaptat...

Because the influenza A virus has an RNA genome, its RNA-dependent RNA polymerase, comprising the PA, PB1, and PB2 subunits, is essential for viral transcription and replication. The binding of RNA primers/promoters to the polymerases is an initiation step in viral transcription. In our current study, we reveal the 2.7 A tertiary structure of the C-terminal RNA-binding domain of PB2 by x-ray cr...

the pb2 protein of influenza a viruses has been shown as host range determinant. in this study pb2gene of five h9n2 isolates from tehran province during the period of 1998-2001 were partially amplifiedand sequenced to do phylogenetic study. iranian isolates could be classified in eurasian lineage according totheir pb2 gene sequences. the pb2 genes of these isolates are different from those of m...

The twelfth edition of the Sardinia Symposium, organised by the IWWG (International Waste Working Group, www.iwwg.eu), was held in Forte Village, Santa Margherita di Pula, Italy from October 5th to 9th. The event was attended by more than 800 participants (researchers, technicians, administrators and operators) from 65 different nations and saw the presentation of 580 scientific papers in 128 g...