background: the main goal of the present study was to set up an axenic cultivation of acanthamoeba and assess the pathogenic ability of t4 genotypes from different clinical and environmental strains of acanthamoeba using two physical assays. methods: sixteen acanthamoeba isolates including 10 environmental and 6 clinical strains were cul-tured axenically. axenic cultivation was performed using ...

A survey was done for the most common yeast isolates from different clinical sources from human and animals in addition to environment samples from soil in El-Fayoum Governorate. The total of tested samples was 802 clinical and environmental samples. They consisted of fifty human vaginal swabs, seventy one human throat swabs, three hundred fifty seven animal vaginal swabs, one hundred seventy n...

Candida dubliniensis is a yeast species that has only recently been differentiated from Candida albicans. C. dubliniensis colonization was initially associated with human immunodeficiency virus (HIV)-positive individuals. Because of the large proportion of AIDS patients in South Africa, we tested the generality of this association by assessing the prevalence of C. dubliniensis colonization amon...

Drought stress in the field has been shown to exacerbate aflatoxin contamination of maize and peanut. Drought and heat stress also produce reactive oxygen species (ROS) in plant tissues. Given the potential correlation between ROS and exacerbated aflatoxin production under drought and heat stress, the objectives of this study were to examine the effects of hydrogen peroxide (H2O2)-induced oxida...

Thermotolerant yeasts are widely considered to be alternative strains traditional for bioethanol production at high temperatures. In this study, thirty-two isolated from lychees were screened thermotolerance, and seven selected isolates identified as Candida tropicalis (isolates H8, H19, H23), Meyerozyma guilliermondii H1 H12) Saccharomyces cerevisiae H10 H18). They tolerated up 45 °C, 12% (v/v...

The MICs and minimal lethal concentrations of four antimycotics, amphotericin B, 5-fluorocytosine, miconazole nitrate, and ketoconazole, were determined for 25 yeast isolates representing species uncommonly implicated in candidiasis. A microdilution procedure was employed with complex and synthetic media. The isolates, in general, were susceptible to the same antimicrobial agents shown to be ef...

The ability of yeast to form biofilms contributes to better survival under stressful conditions. We see the impact of yeast biofilms and "flocs" (clumps) in human health and industry, where forming clumps enables yeast to act as a natural filter in brewing and forming biofilms enables yeast to remain virulent in cases of fungal infection. Despite the importance of biofilms in yeast natural isol...

Abstract Background Plasmodium vivax transmission-blocking vaccines (TBVs) are receiving increasing attention. Based on excellent activities of the PbPH (PBANKA_0417200) and PbSOP26 (PBANKA_1457700) antigens in berghei , their orthologs P. PVX_098655 (PvPH) PVX_101120 (PvSOP26), were selected for evaluation potential as TBVs. Methods Fragments PvPH (amino acids 22–304) PvSOP26 30–272) expressed...

Candida albicans has the ability to change between yeast and hyphal cells and is known to be a virulence property. Efg1gene of C.albicans is as a main transcription factor that plays pivotal roles in biofilm formation The aim of the current study is to investigate the presence of Efg1 gene in Candida albicans isolates from women with vaginal candidiasis and its impact on biofilm formation.We us...