Cryopreservation is the method of choice for long term storage of human PBMCs. This study was designed to compare the different combinations of variables affecting the cryopreservation of PBMCs samples. The viability of PBMCs separated from 2×5 ml peripheral blood samples obtained from 16 healthy adult volunteers, were measured using trypan blue dye exclusion method just before freezing with ...

We propose an efficient computational method to simulate two-phase flow in fractured porous media. Instead of refining the grid to capture the flow along the faults or fractures, we represent them as immersed interfaces with reduced model for the flow and suitable coupling conditions. We allow for non matching grids between the porous matrix and the fracture to increase the flexibility of the m...

A modern numerical scheme for simulation of flow of two immiscible and incompressible phases in inhomogeneous porous media is proposed. The method is based on a combination of the mixed-hybrid finite element (MHFE) and discontinuous Galerkin (DG) methods. The combined approach allows for accurate approximation of the flux at the boundary between neighboring finite elements, especially in hetero...

The ground-state properties of two-dimensional liquid 4 He at zero temperature are studied by means of a quadratic diffusion Monte Carlo method. As interatomic potential we use a revised version of the HFDHE2 Aziz potential which is expected to give a better description of the interaction between helium atoms. The equation of state is determined with great accuracy over a wide range of densitie...

We present a discontinuous Galerkin finite element method for two depth-averaged two-phase flow models. One of these models contains nonconservative products for which we developed a discontinuous Galerkin finite element formulation in Rhebergen et al. (2008) J. Comput. Phys. 227, 1887-1922. The other model is a new depth-averaged two-phase flow model we introduce for shallow two-phase flows th...

Climate change is likely to modify the freezing–thawing cycles in soils and surface waters of permafrost-affected subarctic regions. However, solution chemical composition during ice formation evolution remaining fluids remain very poorly known. Towards a better understanding dissolved (<0.45 µm) organic carbon, as well major trace element behavior permafrost peatland environments, here we p...

Water that remains unfrozen at temperatures below the equilibrium bulk freezing temperature, in the presence of ice, is sometimes called unfreezable or bound. This paper analyses the phenomenon in terms of quantitative measurements of the hydration interaction among membranes or macromolecules at freezing temperatures. These results are related to analogous measurements in which osmotic stress ...

Background Freezing process has destructive effect on different functions of sperm. Therefore, sperm motility was reduced and apoptosis and DNA damage and also levels of reactive oxygen species (ROS) were increased. Insulin-like factor-3 (INSL3) is a member of Relaxin family and directly affects on spermatogenesis and also plays a protective role against apoptosis. Therefore, with due regard to...

A method combining features of front-tracking methods and fixed-domain methods is presented to model dendritic solidification of pure materials. To explicitly track the interface growth and shape of the solidifying crystals, a fronttracking approach based on the level set method is implemented. To easily model the heat and momentum transport, a fixed-domain method is implemented assuming a diff...