An amperometric cytochrome c-based electrode was developed and applied to the quantification of the scavenging capacity of antioxidants. The enzymatic biosensor was constructed by covalently immobilizing both cyt c and XOD on a mercaptoundecanoic acid/mercaptoundecanol mixed SAMmodified screen-printed gold electrode. The concentration which induces a 50% inhibition of the superoxide level (IC50...

The crystal structure of the title water-soluble analogue of vitamin E, trolox amide, C(14)H(19)NO(3), solved and refined against synchrotron diffraction data, contains two mol-ecules in the asymmetric unit. In both molecules, the heterocyclic ring is in a half-chair conformation. The crystal packing features a herring-bone pattern generated by N-H⋯O hydrogen bonds between the hy-droxy and amid...

The use of poly(N-vinyl-2-pyrrolidone) (PVP) hydrogel-supported luminol chemiluminescence (CL) for the automatic determination of hydrogen peroxide and the quantification of the antiradical capacity of Trolox is described. The hydrogel containing luminol and hemin is prepared directly on a 96-well microplate and can be stored for up to 3 months without significant decrease in CL quantum yields....

Madeira wine is produced via spontaneous alcoholic fermentation arrested by ethanol addition. The increasing demand of the market has led to need standardize winemaking process. This study focuses on identifying microbiota indigenous yeasts present during and then evaluates impact selected non-Saccharomyces as pure starter culture (Hanseniaspora uvarum, Starmerella bacillaris, Pichia terricola,...

In recent years, new raw materials have been sought for use in processing. This category certainly includes invasive crayfish Faxonius limosus. One of the problems associated with their is short microbiological shelf life. Therefore, research presented here, an attempt was made to ferment meat strains Lactiplantibacillus plantarum, Lacticaseibacillus rhamnosus, Lactobacillus casei, and yogurt c...

Malpighia emarginata D.C is an important crop of Brazil and significant source antioxidant active compounds with notable health effects. The purpose this work was to study three varieties acerola (Malpighia D.C) at green unripe stage maturity from the São Francisco Valley Northeast Brazil. fruits were evaluated for physico-chemical composition, bioactive compounds, through spectrophotometric an...

The electrochemical properties of three structurally different flavonoids ((+)-catechin, quercetin and rutin) on a glassy carbon electrode were studied at different pH by cyclic, differential pulse and square-wave voltammetry. It was determined that in all investigated compounds oxidation of catechol 3',4'-dihydroxy group on the B-ring (the first oxidation peak) occurs. This process is reversib...

Previous research findings indicate that environmental temperature can influence exercise-induced oxidative-stress responses, although the response to variable temperatures is unknown. The purpose of this study was to investigate the effect of warm, cold, and "neutral," or room, environmental temperatures on the blood oxidative stress associated with exercise and recovery. Participants (N = 12,...

Literature has consistently reported that horticultural wastes including leaves, skin, stones and seeds contain substantial amounts of bioactive compounds. Therefore, this study aims to evaluate antioxidant activity, Total Phenolic Content (TPC) colour parameters in avocado, banana, papaya leaves. Antioxidant activity the leaves was determined using Trolox Equivalent Capacity (TEAC) DPPH radica...