Fusarium wilt caused by Fusarium oxysporum f.sp. Lycopersici is one of the major obstacles to the production of tomato which causes huge losses in tomato products worldwide. In order to increase the tolerance to this disease, a triple structure containing PR1, chitinase and glucanase genes controlled by 35S promoter was transferred to tomato. Eight days after planting on pre-culture me...

In crop research programs that implement transgene-based strategies for trait improvement it is necessary to distinguish between transgene homozygous and hemizygous individuals in segregating populations. Direct methods for determining transgene zygosity are technically challenging, expensive, and require specialized equipment. In this report, we describe a standard PCR-based protocol coupled w...

Background: Canola is an agro-economically oilseed crop. Yield loss due to fungal disease of stem rot caused by Sclerotinia sclerotiorum is a serious problem in canola cultivation. Thaumatin-like proteins are large groups of the pathogenesis-related proteins which provide resistance to the fungal infection in response to invading pathogens and play a key role in plant defense s...

fusarium wilt caused by fusarium oxysporum f.sp. lycopersici is one of the major obstacles to the production of tomato which causes huge losses in tomato products worldwide. in order to increase the tolerance to this disease, a triple structure containing pr1, chitinase and glucanase genes controlled by 35s promoter was transferred to tomato. eight days after planting on pre-culture medium, exp...

Cotton (Gossypium hirsutum L.) seedlings are considered to be sensitive to temperature stress. The exposure of plants to high and low temperatures can result in the production of reactive oxygen species that contribute to diminished plant performance. Plants have mechanisms that metabolize oxidants into less harmful chemicals, and enhancement of antioxidant metabolism has been shown to be benef...

Various regulatory proteins play a fundamental role to manage the healthy plant growth under stress conditions. Differential display reverse transcriptase PCR and random amplification of cDNA ends (RACE) was used to explore the osmotic stress-responsive transcripts. We identified and characterized the salt stress-responsive R2R3 type RMYB transcription factor from Hibiscus sabdariffa which has ...

Over the past decade RNA interference (RNAi) technology has emerged as a successful tool not only for functional genomics, but in planta expression of short interfering RNAs (siRNAs) that could offer great potential for insect pest management. The diet of insects feeding exclusively on phloem sieves contains water and sugars as main components, and the uptake of the liquid food greatly depends ...

Evolving levels of resistance in insects to the bioinsecticide Bacillus thuringiensis (Bt) can be dramatically reduced through the genetic engineering of chloroplasts in plants. When transgenic tobacco leaves expressing Cry2Aa2 protoxin in chloroplasts were fed to susceptible, Cry1A-resistant (20,000- to 40,000-fold) and Cry2Aa2-resistant (330- to 393-fold) tobacco budworm Heliothis virescens, ...

Verticillium wilt caused by soilborne fungus Verticillium dahliae could significantly reduce cotton yield. Here, we cloned a tomato Ve homologous gene, Gbve1, from an island cotton cultivar that is resistant to Verticillium wilt. We found that the Gbve1 gene was induced by V. dahliae and by phytohormones salicylic acid, jasmonic acid, and ethylene, but not by abscisic acid. The induction of Gbv...

Flax (Linum usitatissimum L.) is an annual plant cultivated mainly for its fibres. Although the whole genus contains about 230 species, only L. usitatissimum retains an important commercial use, serving as a fibre source for the textile industry. Flax fibres are less elastic than cotton fibres, and thus their use in textile production is more limited. However, the climate and soil quality in ce...