Spore formation is a sophisticated mechanism by which some bacteria survive conditions of stress and starvation by producing a multilayered protective capsule enclosing their condensed DNA. Spores are highly resistant to damage by heat, radiation, and commonly employed antibacterial agents. Previously, spores have also been shown to be resistant to photodynamic inactivation using dyes and light...

b-Cyclodextrin forms 1:1 inclusion complexes with methylene blue, azure A, toluidine blue, resorcinol blue, neutral red, safranine T, indigo carmine and acridine orange in aqueous media. The formation constants are determined by differential pulse polarography and spectrophotometry. The supramolecular interaction in the inclusion complexes can be employed to immobilize dyes on an electrode. Thi...

IMPORTANCE Clinical features are unreliable for distinguishing ocular surface squamous neoplasia (OSSN) from benign conjunctival lesions. OBJECTIVE To evaluate the adverse effects, accuracy, and interobserver variation of toluidine blue 0.05% vital staining in distinguishing OSSN, confirmed by histopathology, from other conjunctival lesions. DESIGN, SETTING, AND PARTICIPANTS Cross-sectional...

Background: Varicocele occurs in approximately 15% to 20% of the male population and it is the most common cause of poor semen parameters. It has been demonstrated that patients with varicocele have poor chromatin packing, DNA damage and nuclear anomalies than the healthy men. Therefore, the aim of this study was to evaluate sperm chromatin integrity and semen parameters in these patients after...

Serratia marcescens was isolated on a new medium-commercial deoxyribonuclease agar with the addition of cephalothin (1,000 mug/ml) and Toluidine Blue (1,000 mug/ml). It was detected in water samples even though it comprised only 0.1 to 0.0001% of the total bacterial population.

Numerous tools and techniques such as vital stain, autofluorescence, exfoliative cytology, histomorphometry, etc. were explored in the detection of dysplasia, but have variable sensitivity specificity. The present study evaluated effect toluidine blue increasing accuracy histomorphometry analysis dysplasia OPMDs on cytology. An experimental, observational, cross-sectional research was performed...

Introduction: Morphological differentiation between reactive mesothelial proliferation and metastatic carcinoma cells may be extremely difficult in conventional centrifuge deposit smears stained with papanicolaou and romanowsky dyes. In the present study immunocytochemistry and morphometric analysis were performed on cytospin preparation of effusion categorized as atypical/suspicious on toluidi...

background: biofilm infections are a major challenge in medical practice. bacteria that live in a biofilm phenotype are more resistant to both antimicrobial therapy and host immune responses compared to their planktonic counterparts. so, there is need for new therapeutic strategies to combat these infections. a promising approach [known as photodynamic inactivation (pdi)] to kill bacteria growi...