Objective To evaluate the ability of the eluate from fibrin-rich plasma (FRP) membrane to induce proliferation and differentiation of isolated human adipose-derived stem cells (ASCs) into chondrocytes. Method FRP membranes were obtained by centrifugation of peripheral blood from two healthy donors, cut, and maintained in culture plate wells for 48 h to prepare the fibrin eluate. The SCATh wer...

A morphological and quantitative study was performed on the respiratory epithelium of human distal airways in 20 lungs removed at operation for the treatment of carcinoma. Using 1 micron araldite sections stained with Toluidine Blue for light microscopy, with further electron microscopic study where required, we have described mast cells in the epithelium of bronchioles, terminal bronchioles, a...

An autoradiographic study was made using S(35)-sulfate for the localization, distribution, and variation in the mucopolysaccharide content of the femoral periosteum of rats from birth to old age. The mucopolysaccharides were also studied histochemically, using toluidine blue O, Rinehart and Abu'l-Haj's colloidal iron method, and the periodic acid-Schiff reaction, before and after hyaluronidase ...

In a toluidine blue dye (British Drug Houses, batch no. 651880), we report a purple chromato­ graphic fraction (Rf—0,55) with a spectral absorption peak at 622 m/u, which has the property to induce immediate bright yellow fluorescence (primary inducible fluorescence) in cytoplasmic gra­ nules of tumor cells. The cells are unfixed, supravital stained and examined by fluorescence micro­ scopy. Th...

Four viruses were irreversibly photosensitized by 4 × IO-SM-ethidium chloride but not by more dilute solutions. Photosensitization was greatest with vaccinia, pseudorabies, herpes tamarinus and reoviruses, intermediate with western equine encephalitis virus, and least with fowl plague and Newcastle disease viruses. Pseudorabies virus was also inactivated by ethidium chloride at 37 ° in the dark...

This aim of this study was to assess the ability of manual or rotary instrumentation associated with photodynamic therapy (PDT) to reduce Enterococcus faecalis using three combinations of light/photosensitizers: toluidine blue O/laser, fuchsin/halogen light and fuchsin/LED. Twenty deciduous molars were selected and contaminated with Enterococcus faecalis (McFarland 0.5 scale). Working length de...

Indoor fungi growth is an increasing home health problem as our homes are more tightly sealed. One thing that limits durability of the antifungal agents is the scarcity of reactive sites on many surfaces to attach these agents. In order to increase graft yield of photosensitizers to the fabrics, poly(acrylic acid-co-styrene sulfonic acid-co-vinyl benzyl rose bengal or phloxine B) were polymeriz...

Mouse spermatozoa were treated with trypaflavine, toluidine blue or nitrogen mustard before fertilization in attempts to destroy the sperm chromosomes and so induce haploid gynogenetic development of the egg. High concentrations of either chemical decreased the activity of spermatozoa. Trypaflavine and toluidine blue had a photodynamic action on spermatozoa and lowered the fertilization rate; n...

Electron microscopy was carried out on sections through tissue mast cells from the peritoneal fluid of rats and hamsters, either untreated, x-irradiated, or injected with toluidine blue, protamine sulfate, or stilbamidine. Mast cells from untreated animals have large nuclei and are filled with densely packed, cytoplasmic granules. The latter possess a distinct boundary and an internal structure...

Peri-implantitis is considered to be a multifactorial process involving bacterial contamination of the implant surface. A previous study demonstrated that a combination of toluidine blue O (100 microgram/ml) and irradiation with a diode soft laser with a wavelength of 905 nm results in an elimination of Porphyromonas gingivalis (P. gingivalis), Prevotella intermedia (P. intermedia), and Actinob...