Thin layer chromatography (TLC) is a useful technique for the separation and identification of compounds in mixtures. TLC is used routinely to follow the progress of reactions by monitoring the consumption of starting materials and the appearance of products. Commercial applications of TLC include the analysis of urine for evidence of "doping", the analysis of drugs to establish purity or ident...

The present study reports the Gas Chromatography-Mass Spectrometry (GC-MS) evaluation of the hexanes and dichloromethane fractions from extracts of the red alga Centroceras clavulatum (C. Agardh) Montagne. Twenty three compounds were identified, totaling ca. 42% of both fractions (0.18 g mass extract). The main constituents of the fractions were hexadecanoic acid (17.6%) and pentadecanoic acid ...

Chemical composition and antimicrobial activity of the hydrodistilled essential oils from aerial parts and roots of Aster subulatus Michx. (Asteraceae) from Turkey were investigated. The essential oils of A. subulatus were analyzed by combination of GC-FID and GC-MS. Twenty-nine components comprising 79.0% of the essential oil of the roots and forty-nine components comprising 89.3% of the essen...

The preliminary phytochemical screening of ethanolic extracts Averrhoa carambola (A. carambola) leaves were done using the standard protocol. findings analysis exhibited occurrence Carbohydrates, Alkaloids, Steroids, Tannins, Vitamin C and flavonoids by Folin-Ciocalteu technique aluminum chloride colorimetric technique, respectively, whole phenolic or flavonoid amounts evaluated found 194.48±0....

BACKGROUND The current study was conducted to evaluate the antioxidant, analgesic, antihyperglycemic, neuropharmacological and antidiarrheal activities of ethanolic extract of Lepisanthes rubiginosa L. leaves in different experimental models. METHODS Quantitative and qualitative analysis were done by TLC (thin layer chromatography) and DPPH (1,1-diphenyl-2-picrylhydrazyl) free radical scaveng...

background & aim:oxidative stresses caused by free radicals are known to promote many diseases such as inflammation and cancer; therefore, any plant product that can inhibit free radical production can play an important role in preventing diseases. we aimed to compare the fracture rate and the scavenging activity of free radicals, and the antioxidant composition of rolb-transformed hairy root o...

BACKGROUND This study evaluated the antibacterial activity of some plants used in folklore medicine to treat diarrhoea in the Eastern Cape Province, South Africa. METHODS The acetone extracts of Acacia mearnsii De Wild., Aloe arborescens Mill., A. striata Haw., Cyathula uncinulata (Schrad.) Schinz, Eucomis autumnalis (Mill.) Chitt., E. comosa (Houtt.) Wehrh., Hermbstaedtia odorata (Burch. ex ...

Objective: The aims of the present study were to screen actinobacteria with high potential ability produce secondary metabolites that have inhibitory activity against plant pathogenic fungi, Magnaporthe oryzae. Production was analysis by thin-layer chromatography and bioautography assay.
 Methods: Screening selection Streptomyces sp. morphological, cultural, physiological, biochemical char...

We report the use of TLC image analysis software, JustTLC, for identification and quantitative evaluation of the photoproducts generated by the irradiation of Chlorpromazine water solutions with a 266 nm laser beam. The percent concentrations resulting for photoproducts from TLC analysis were compared with the relative concentrations of those identified by LC-TOF/MS measurements. The spots on t...

In the course of biosynthesis of cobamides and their related compounds, a variety of intermediates can be found in addition to the final products. For the isolation, identification, and characterization of these substances, column chromatography, paper electrophoresis and physicochemical procedures such as ultraviolet and visible spectrophotometry have been employed. Sometimes, microbiological ...