Renal transplantation is one of the best choices for the treatment to the end stage renal disease. However, the shortage of the donor organs is a worldwide challenge, and the huge demand for transplantation forced the widely use of organs from expanded criteria donors or donation after cardiac death. Kidneys from these donors are highly sensitive to ischemia reperfusion (IR) injury, which might...

This study was conducted to measure cell-mediated immune response in healthy Epstein Barr virus (EBV)-seropositive individuals using a tissue culture "growth inhibition" assay (regression assay) where peripheral blood lymphocytes (PBLs) were tested for their ability to inhibit the outgrowth of the autologous lymphoblastoid cell lines (LCLs). Inhibition of the outgrowth of the autologous LCLs wa...

BACKGROUND It is known that tissue macrophages derive not only from blood monocytes but also from yolk sac or fetal liver, and the tissue of residence guides their function. When isolated, they lose tissue specific signatures, hence studies of human macrophages should be ideally done directly in the tissue. The aim of this study was to investigate directly in human lung tissue the polarization ...

Despite efforts to increase the supply of organs from living donors, most kidney transplants performed in Australia still come from deceased donors. The age of these donated organs has increased substantially in recent decades as the rate of fatal accidents on roads has fallen. The Organ and Tissue Authority in Australia is therefore looking to design a new mechanism that better matches the age...

THE separation and purification of human islets from the pancreas of pediatric-age donors has been considered difficult if not impossible because of easy fragmentation of the islets and/or their incomplete separation from the enclosing acinar tissue (embedding). I.:! The purpose of this study was to develop a method to separate and purify large numbers of viable human islets from pediatric dono...

D-dopachrome tautomerase (D-DT/MIF-2) is a member of the macrophage migration inhibitory factor (MIF) cytokine superfamily, and a close structural homolog of MIF. MIF and D-DT have been reported to be involved in obesity, but there is little known about the regulation of D-DT in adipose tissue inflammation and wound healing. Subcutaneous adipose tissue was collected from 54 healthy donors and 2...

We discovered that induced pluripotent stem cell (iPSC) clones generated from aged tissue donors (A-iPSCs) fail to suppress oxidative phosphorylation. Compared to embryonic stem cells (ESCs) and iPSCs generated from young donors (Y-iPSCs), A-iPSCs show poor expression of the pluripotent stem cell-specific glucose transporter 3 (GLUT3) and impaired glucose uptake, making them unable to support t...

OBJECTIVE To investigate whether the accident and emergency department (A&E) is a potential source of tissues for donation, from non-heart beating donors (NHBDs). METHODS A telephone survey of 30 A&E departments was conducted to determine current tissue harvesting practices from NHBDs. The potential number of tissue donors in our own medium sized district general hospital A&E department was e...

Over the last few decades, polymers and their composites have shown a lot of promises in providing more viable alternatives to surgical procedures that require scaffolds implants. With advancement biomaterial technologies, it is possible overcome limitations current methods, including auto-transplantation, xeno-transplantation, implantation artificial mechanical organs used treat musculoskeleta...

BACKGROUND To report the feasibility of intraoperative spectral domain optical coherence tomography (OCT) using the RESCAN 700 for the visualization and evaluation of precut Descemet's stripping automated endothelial keratoplasty (DSAEK) and prestripped Descemet's membrane endothelial keratoplasty (DMEK) donor tissue. METHODS Precut DSAEK (n = 11), prestripped DMEK (n = 4) preserved in a view...