نتایج جستجو برای: thiol alkylating peg reagent

تعداد نتایج: 48611  

Journal: :Biomacromolecules 2015
Jing Jing Audrey Fournier Anna Szarpak-Jankowska Marc R Block Rachel Auzély-Velty

In this work, cell-responsive polysaccharide hydrogels were prepared by a simple procedure based on the sequential bioconjugation and cross-linking of the polysaccharide backbone with bioactive peptides and poly(ethylene glycol)-bis(thiol) (PEG-(SH)2), respectively. Using thiol-ene reactions, we successfully functionalized hyaluronic acid (HA) and carboxymethylcellulose (CMC) with short and lon...

Journal: :Journal of bacteriology 1970
R Wax E Rosenberg N S Kosower E M Kosower

Oxidation of glutathione within Escherichia coli cells by diamide, (CH(3))(2)NCON=NCON(CH(3))(2), stops growth but does not cause cell death. Normal growth rates are resumed after periods which vary in length according to the diamide concentration. Consumption of excess reagent with added glutathione quickly reverses the inhibition. Another thiol-oxidizing agent, azoester, C(6)H(5)N=NCOOCH(3), ...

2017
Yong Xia Sha Ding Yuejun Liu Zhengjian Qi

We demonstrated here a facile and efficient synthesis of polyhedral oligomeric silsesquioxane-based amphiphilic polymer by thiol-ene click chemistry. The properties of polyhedral oligomeric silsesquioxane (POSS)–PEG amphiphilic polymers were studied in detail by a combination of 1H NMR, 13C NMR, 29Si NMR FT-IR, GPC, and TG analysis. The newly-designed thiol-ene protocol obtains only anti-Markov...

2016
Barbara Bernardim Pedro M S D Cal Maria J Matos Bruno L Oliveira Nuria Martínez-Sáez Inês S Albuquerque Elizabeth Perkins Francisco Corzana Antonio C B Burtoloso Gonzalo Jiménez-Osés Gonçalo J L Bernardes

Maleimides remain the reagents of choice for the preparation of therapeutic and imaging protein conjugates despite the known instability of the resulting products that undergo thiol-exchange reactions in vivo. Here we present the rational design of carbonylacrylic reagents for chemoselective cysteine bioconjugation. These reagents undergo rapid thiol Michael-addition under biocompatible conditi...

2014
Zuzana Gaziova Volker Baumann Anna-Maria Winkler Johannes Winkler

The therapeutic application of siRNA suffers from poor bioavailability caused by rapid degradation and elimination. The covalent attachment of PEG is a universal concept to increase molecular size and enhance the pharmacokinetic properties of biomacromolecules. We devised a facile approach for attachment of PEG molecules with a defined molecular weight, and successful purification of the result...

Journal: :Advanced functional materials 2010
Hye Jung Han Rangaramanujam M Kannan Sunxi Wang Guangzhao Mao Juan Pedro Kusanovic Roberto Romero

Dendrimers, with their well-defined globular shape and a high density of functional groups, are ideal nanoscale materials for templating sensor surfaces. This work exploits dendrimers as a versatile platform for capturing biomarkers with improved sensitivity and specificity. Synthesis, characterization, fabrication, and functional validation of the dendrimer-based assay platform are described. ...

2008
Sunny Shah He Zhu Jun Yan Stanislav Verkhoturov Alexander Revzin

In this paper, we demonstrate the use of individually addressable microelectrodes for cell sorting and cell micropatterning applications. Microelectrodes were modified with cell adhesive or non-adhesive molecules and then electrically stimulated to selectively adsorb or desorb proteins and/or mammalian cells. The switching of the surface properties was achieved by the electrochemical desorption...

Journal: :Biomacromolecules 2012
Lorenzo Albertazzi Frauke M Mickler Giovanni M Pavan Fabrizio Salomone Giuseppe Bardi Mariangela Panniello Elizabeth Amir Taegon Kang Kato L Killops Christoph Bräuchle Roey J Amir Craig J Hawker

Hybrid dendritic-linear block copolymers based on a 4-arm poly(ethylene glycol) (PEG) core were synthesized using an accelerated AB2/CD2 dendritic growth approach through orthogonal amine/epoxy and thiol-yne chemistries. The biological activity of these 4-arm and the corresponding 2-arm hybrid dendrimers revealed an enhanced, dendritic effect with an exponential increase in cell internalization...

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