Thiocyanate is a C1 compound containing carbon, nitrogen, and sulfur. It is a (by)product in a number of natural and industrial processes. Because thiocyanate is toxic to many organisms, including humans, its removal from industrial waste streams is an important problem. Although a number of bacteria can use thiocyanate as a nitrogen source, only a few can use it as an electron donor. There are...

BACKGROUND AND AIM: The prevalence of tobacco use and its associated mortality is increasing worldwide. Biomarkers in saliva, such as thiocyanate levels, have been shown to be a suitable indicator for smoking. The present study is conducted to determine the thiocyanate levels in saliva and compare them among tobacco (smoking and smokeless) users, passive smoker...

The present study was undertaken to assess the state of iodine nutrition of the representative population, consumption pattern of cyanogenic plant food that generally interfere with iodine nutrition, and to evaluate whether there is any interrelation between iodine and thiocyanate intake and their excretion pattern among school children in goiter endemic subHimalayan tarai region. A total of 56...

An alkali-tolerant bacterium, Pseudomonas aeruginosa STK 03 (accession number KR011154), isolated from an oil spill site, was evaluated for the biodegradation of free cyanide and thiocyanate under alkaline conditions. The organism had a free cyanide degradation efficiency of 80 and 32 % from an initial concentration of 250 and 450 mg CN-/L, respectively. Additionally, the organism was able to d...

Patients in clinical practice often falsely report their smoking status. To see if this was so in occupational surveys we have validated smoking histories (using a serum thiocyanate assay) in 206 randomly sampled office workers who completed a smoking questionnaire administered by a doctor. Past and present cigarette consumption was determined with an assessment of exposure to passive cigarette...

Pd(II) and Pt(II) complexes of trimethoprim and pyrimethamine were synthesized and characterized by elemental analysis, UV-Vis, FTIR, and NMR spectroscopy. The complexes are formulated as four coordinate square planar species containing two molecules of the drugs and two chloride or thiocyanate ions. The coordination of the metal ions to the pyrimidine nitrogen atom of the drugs was confirmed b...

A draft genome sequence of a neutrophilic halophile capable of thiocyanate degradation, Thiohalobacter thiocyanaticus FOKN1, was determined using a PacBio RSII sequencer. A 3.23-Mb circular genome sequence was assembled, in which 3,026 gene-coding sequences, 45 tRNAs, and 1 rrn operon were annotated.

The reagent described is 5.5 M in perchioric acid and 3.3 M in urea. It contains ferric ions, mercuric thiocyanate, mercuric ions (from mercuric perchiorate), and mercuric chloride. Serum dissolves directly in this reagent to yield a clear, reddish solution. When chloride ions are added, they combine first with the free mercuric ions, and then with some of the mercuric ions from the mercuric th...

Measurements of the electrophoretic mobilities of insulin reported by Hall (1, 2) are limited to regions higher than pH 7 and lower than pH 4.5, since the protein is practically insoluble in common buffer solutions of the intermediate pH range. While it has been possible to determine the elect.rophoretic mobility of adsorbed insulin and of suspensions of insulin crystals in the isoelectric rang...

We describe a kinetic enzymic method for serum bicarbonate analysis, using wheat germ phosphoenolpyruvate carboxylase (EC 4.1.1.31) coupled through oxaloacetate reduction with NADH in the presence of malate dehydrogenase (EC 1.1.1.37). Inhibition with potassium thiocyanate yielded first-order kinetics with respect to bicarbonate over the concentration range of 0-45 mmol/L. The inhibitor was cho...