نتایج جستجو برای: thiazoly blue tetrazolium

تعداد نتایج: 75825  

Journal: :Bioscience, biotechnology, and biochemistry 2008
Saikat Chakraborty Makiko Sakka Tetsuya Kimura Kazuo Sakka

A small enzyme showing diaphorase activity was purified from culture supernatant of Clostridium kluyveri and its N-terminal amino acid sequence was determined. This sequence identified a gene (diaA) encoding a protein (DiaA) of 229 amino acids with a predicted molecular weight of 24,981 in the genomic DNA sequence database of C. kluyveri constructed by the Research Institute of Innovative Techn...

Journal: :Antimicrobial agents and chemotherapy 1985
H A Shoeb B U Bowman A C Ottolenghi A J Merola

During the course of horseradish peroxidase-mediated oxidation of either o-dianisidine or 2-2'-azino-di(3-ethyl-benzthiazoline-6-sulfonic acid) (ABTS), no O2 consumption took place. When isonicotinic acid hydrazide (isoniazid) (INH) was included in the reaction mixture, O2 was consumed in amounts linearly related to the INH concentration. Nicotinic acid hydrazide at equimolar concentrations ind...

Journal: :The Biochemical journal 1982
M R Coudray G Canevascini H Meier

An extracellular enzyme from culture filtrates of Sporotrichum (Chrysosporium) thermophile (A.T.C.C. 42 464) after growth on cellulose or cellobiose was shown to oxidize cellobiose to cellobionic acid in vitro. Lactose and cellodextrins were also efficiently oxidized, but the enzyme was not active against most mono- and di-saccharides. Several redox substances could act as electron acceptors, b...

2012
Jyoti Mahajan Rakesh Kumar Rajbir Kaur Saroj Arora

Xanthine oxidase is a member of molybdoenzyme family and has catalytic role in purine degradation, metabolizing hypoxanthine and xanthine to uric acid with the generation of superoxides. In the present study, microtitre plate based colorimetric assay was used to screen the xanthine oxidase producing microorganisms from the soil treated with hypoxanthine with different concentrations (2mM, 4mM, ...

Journal: :The Journal of biological chemistry 1989
T Abe M Murakami T Sato M Kajiki M Ohno R Kodaira

Human macrophage differentiation inducing factor (DIF) can induce differentiation of human myeloid leukemic cells into macrophage-like cells in vitro. A procedure is described for purification of DIF from serum-free human monocytic leukemia THP-1 cell-conditioned medium. The procedure included concentration of a conditioned medium by ultrafiltration, lentil lectin-Sepharose affinity chromatogra...

2009
Amit Agarwal U. C. Banerjee

Xanthine oxidase is a highly versatile and ubiquitous complex molybdoflavoprotein, which controls the rate limiting step of purine catabolism pathway. Microbial xanthine oxidase can be used to address a number of questions presently not feasible with the eukaryotic enzymes. In the present study, a high-throughput microtitre plate-based colorimetric assay for xanthine oxidase producing microorga...

2015
A Dyson A Lach M Singer

Methods Ventilated, anaesthetized rats (4/group) were instrumented with vascular lines for drug/fluid administration. Following thoracotomy, the left anterior descending (LAD) coronary artery was ligated for 30 min. Just prior to reperfusion, animals received i.v. MGC-0109 (10 mg/kg bolus) or n-saline. MGC-0109-treated animals received a further 2.5 mg/kg infusion over 15 min. Control animals r...

2004
Yoshio Honma Takashi Kasukabe Motoo Hozumi Susumu Tsushima Hiroaki Nomura

Alkyl-lysophospholipids are synthetic analogs of naturally occurring lysophospholipids. The effects of these compounds on cell proliferation and differentiation of cultured human (HL60) and mouse (M1) myeloid leukemia cells were studied. Both cell lines were induced to differentiate into morpholog ically and functionally mature granulocytes and macrophages by incubation with a wide variety of t...

Journal: :Cancer research 1981
Y Honma T Kasukabe M Hozumi S Tsushima H Nomura

Alkyl-lysophospholipids are synthetic analogs of naturally occurring lysophospholipids. The effects of these compounds on cell proliferation and differentiation of cultured human (HL-60) and mouse (M1) myeloid leukemia cells were studied. Both cell lines were induced to differentiate into morphologically and functionally mature granulocytes and macrophages by incubation with a wide variety of t...

Journal: :Zeitschrift fur Naturforschung. C, Journal of biosciences 2002
Gerhard Erkel Alexandra Gehrt Timm Anke Olov Sterner

The HL-60 cell line, derived from a patient with acute promyelocytic leukemia, is a widely used model system to study the cellular and molecular events involved in differentiation of leukemic cells. In a screening for inducers of differentiation of HL-60 cells, cultures of Gliocladium strain 4-93 were found to produce Sch 52900, a previously isolated diketopiperazine (Chu et al,. J Antibiotics ...

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