This technical note describes kinetic fluorescence as an analytical technique to quantify non-fluorescent species. The technique is applied to the determination of thiamine (vitamin B1) in solution. Fluorescence intensity is monitored as thiamine converts to fluorescent thiochrome, and a relationship is established between the rate of increase of intensity and the concentration of thiamine. The...

The normal concentration of thiamine in the blood of human subjects has been determined by several investigators. In most instances an average value of the order of 8.0 to 9.0 y of thiamine per 100 ml. of whole blood has been found (1, 2). Goodhart and Sinclair (2) have reported a value of 5.7 y per 100 ml. for the ox and the considerably higher value of 20.2 y per 100 ml. for the pigeon. Schul...

Thiamine, a water-soluble vitamin, is essential for normal cellular functions, growth and development. Thiamine deficiency leads to significant clinical problems and occurs under a variety of conditions. To date, however, little is known about the mechanism of thiamine absorption in the native human small intestine. The objective of this study was, therefore, to characterize the mechanism of th...

One obvious explanation for the effect of high fat diets in lowering the requirement for thiamine has been that fat metabolism does not require thiamine, while carbohydrate metabolism does. This explanation finds considerable support in one of the known functions of thiamine pyrophosphate. According to current theory, carbohydrate is oxidized to pyruvate, and pyruvate must be decarboxylated for...

Candida species are associated with an increasing number of life-threatening infections (candidiases), mainly due to the high resistance of these yeast-like fungi to antifungal drugs and oxidative stress. Recently, thiamine (vitamin B1) was found to alleviate stress responses in Saccharomyces cerevisiae; however, thiamine influence on defense systems in pathogenic fungi has never been investiga...

Thiamine (vitamin B1) is required in the diet of animals, and thiamine deficiency leads to diseases such as beri-beri and the Wernicke-Korsakoff syndrome. Dietary thiamine (vitamin B1) consists mainly of thiamine pyrophosphate (TPP), which is transformed into thiamine by gastrointestinal phosphatases before absorption. It is believed that TPP itself cannot be transported across plasma membranes...

Although the reduced folate carrier RFC1 and the thiamine transporters THTR-1 and THTR-2 share approximately 40% of their identity in protein sequence, RFC1 does not transport thiamine and THTR-1 and THTR-2 do not transport folates. In the present study, we demonstrate that transport of thiamine monophosphate (TMP), an important thiamine metabolite present in plasma and cerebrospinal fluid, is ...

The present study was prompted by the observation that ingestion of large amounts of nicotinic acid resulted in a relatively large increase in the apparent excretion of thiamine by human subjects maintained on a constant low intake of thiamine. Although the daily excretion of thiamine had been at a fairly constant level, between 20 and 30 y for several months, after administration of 300 mg. of...

OBJECTIVE Thiamine is an essential component of cellular metabolism, and lack of this vitamin results in a potentially life-threatening biochemical lesion. The stress of surgery and critical disease depletes electrolytes, minerals, and essential biochemical substrates. We hypothesized that critical illness (represented by major surgery) would result in decreased thiamine levels over time. MET...

Thiamine, riboflavin and niacin are essential nutrients play vital role in oxidation-reduction reaction during energy production the living cell of body. Requirement these vitamins must be met through diet as human body cannot synthesize thiamine riboflavin. Small amount is synthesized but major requirement external diet. Jute leaves rich different minerals. Different varieties fresh jute well ...