Background: Fibroblasts are mesenchymal cells that can be readily cultured in the laboratory and play a significant role in epithelialmesenchymal interactions, secreting various growth factors and cytokines that have a direct effect on epidermal proliferation, differentiation and formation of extracellular matrix. They have been incorporated into various tissue-engineered and used for a variety...

Calluses from seasonal mature shoots of Taxus globosa Schltdl. were subcultured in new medium containing 1 mg l -1 NAA, 0.5 mg l -1 2, 4-D, 0.025 mg l -1 BA. Calluses growing for 30 and 40 days in a medium added with 0.1-5.0 mg l -1 of chlorocholine chloride (CCC) were harvested for determination of taxol, phenolic compounds, and for polyphenol oxidase (PPO) activity. The results showed that by...

The aim of this paper was to determine whether the interaction between IGF, IGFBP, and VN modulates the functions of porcine EOE cells. Enamel organs from 6-month-old porcine third molars were dissociated into single epithelial cells and subcultured on culture dishes pretreated with VN, IGF-I, and IGFBP-3 (IGF-IGFBP-VN complex). The subcultured EOE cells retained their capacity for ameloblast-r...

Glossina morsitans were infected with two cloned stocks of T. congolense. The proboscides, foreguts and midguts of infected flies were then used as sources of trypanosomes in vitro at 28 degrees C in the presence of bovine dermal collagen explants. Cultures were established in which trypanosomes differentiated into adhering colonies of epimastigote forms which could then be maintained and subcu...

this research was aimed to investgate the effect of light quality on micropropagation of pear rootstock oh×f333. first, single nodes explants were cultured in modified ql medium containing ba at four levels (0, 0.5, 1, and 2 mg/l-1) and iaa at three levels (0, 0.1, and 1 mg/l-1) and were evaluated for shoot proliferation. then, plantlets subcultured in modified ql medium containing 1 mg/l-1 ba ...