High-solids incubations were performed to enrich for microbial communities and enzymes that decompose rice straw under mesophilic (35°C) and thermophilic (55°C) conditions. Thermophilic enrichments yielded a community that was 7.5 times more metabolically active on rice straw than mesophilic enrichments. Extracted xylanase and endoglucanse activities were also 2.6 and 13.4 times greater, respec...

Thermophilic treatment is one of the advanced treatment technologies used for the purification of high-strength wastewaters. Thermophilic systems are conventionally used for sludge treatment, but their ability to reduce COD to a great extent with sludge generation one-tenth when compared to the other wastewater treatment technologies, have favored its application for wastewater purification. Mo...

MOTIVATION Understanding the basis of protein stability in thermophilic organisms raises a general question: what structural properties of proteins are responsible for the higher thermostability of proteins from thermophilic organisms compared to proteins from mesophilic organisms? RESULTS A unique database of 373 structurally well-aligned protein pairs from thermophilic and mesophilic organi...

In this study, we used features that can be extracted from protein sequences to discriminate mesophilic, thermophilic and hyper-thermophilic proteins. Amino acid frequency, dipeptide amino acid frequency and physical-chemical features are used in this study. The effect of mentioned features on proposed discrimination algorithm was evaluated both separately and in combination. Statistical method...

Table 1 Thermophilic moulds are a potential reservoir of thermozymes which find potential applications in various biotechnological processes (Singh , 2016; Singh, 2016; Maheshwari 2000; Johri 1999). Enzymes from thermophilic fungi often tolerate higher temperatures than enzymes from their mesophilic counterparts and some show stability even at 70-80°C (Singh 2016; Singh, 2016; Johri 1999). It h...

Recent investigations on the stability of proteins have demonstrated various structural factors, but few have considered sequence factors such as protein motifs. These motifs represent highly conserved regions and describe critical regions that may only exist on proteins that remain functional at high temperatures. This investigation presents a method for identifying and comparing corresponding...

This study aims to determine suitable start-up conditions and inoculum sources for thermophilic anaerobic digestion. Within days of incubation MSW at 55°C, methane was produced at a high rate. In an attempt to narrow down which components of typical MSW contained the thermophilic methanogens, vacuum cleaner dust, banana peel, kitchen waste, and garden waste were tested as inoculum for thermophi...

Microbial diversity in a thermopilic UASB reactor producing biogas from palm oil mill effluent (POME) was investigated. The PCR-based DGGE analysis showed that the microbial population profiles changed with the temperature transition from mesophilic to thermophilic conditions. Acetotrophic and hydrogenotrophic methanogens were found in all three phases: the seed sludge, transition from mesophil...