Pectin methylesterase (PME) is an important enzyme that can alter the texture of potato products during processing and storage. To manage textural properties based products, low and/or high temperature blanching be used to promote or inactivate PME, respectively. However, many cultivars being for may contain PMEs with a different thermal stability. Therefore, inactivation PME extract from three...

Penicillolysin is a member of the clan MX and the family of M35 proteases. The enzyme is a thermolabile Zn(2+)- protease from Penicillium citrinum with a unique substrate profile. We expressed recombinant penicillolysin in Aspergillus oryzae and generated several site-directed mutants, R33E/E60R, A167E and T81P, with the intention of exploring thermal stabilization of this protein. We based our...

Paradoxical absence of a pro-thrombotic phenotype in a Mouse model of severe hyper-homocysteinemia. The study by Eğin and Akar [1] entitled, First observation of MTHFR 678C-A (Ala 222 Ala) single nucleotide polymorphism, in the recent issue of the Journal (2012; 29: 204-5) requires questioning its importance to thrombosis in the light of Dayal et al.'s findings in a mouse model [2]. I used to t...

Kinetic properties and thermal stabilities of Hoplias malabaricus liver and skeletal muscle unfractionated malate dehydrogenase (MDH, EC 1.1.1.37) and its isolated isoforms were analyzed to further study the possible sMDH-A* locus duplication evolved from a recent tandem duplication. Both A (A1 and A2) and B isoforms had similar optima pH (7.5-8.0). While Hoplias A isoform could not be characte...

The generally accepted view that alpha staphylolysin is thermolabile has recently been questioned by Smith.2" He further demonstrated that some samples of toxin were rendered inactive by heating at 60° C. for 30 minutes, yet retained considerable lytic activity when heated for the same period at 80°. The thermostability of staphylolysin during certain stages of its evolution in shake cultures, ...