Culture methods were developed for the concurrent recovery of Escherichia coli O157:H7 and Salmonella from bovine carcass, hide, and fecal samples. Several enrichment conditions were tested for the overall growth of pure cultures; tryptic soy broth for 2 h at 25 degrees C and then for 6 h at 42 degrees C was the protocol selected for use. Immunomagnetic separation (IMS) was incorporated for sen...

Chemical dissimilarity of tellurium oxide with silica glass increases phase separation and crystallization tendency when mixed and melted for making a glass. We report a novel technique for incorporating an Er(3+)-doped tellurite glass composition into silica substrates through a femtosecond (fs) laser generated plasma assisted process. The engineered material consequently exhibits the spectros...

This work shows that the recently described Escherichia coli BtuE peroxidase protects the bacterium against oxidative stress that is generated by tellurite and by other reactive oxygen species elicitors (ROS). Cells lacking btuE (ΔbtuE) displayed higher sensitivity to K(2)TeO(3) and other oxidative stress-generating agents than did the isogenic, parental, wild-type strain. They also exhibited i...

The mechanisms used by Shiga toxin (Stx)-producing Escherichia coli to adhere to epithelial cells are incompletely understood. Two cosmids from an E. coli O157:H7 DNA library contain an adherence-conferring chromosomal gene encoding a protein similar to iron-regulated gene A (IrgA) of Vibrio cholerae (M. B. Goldberg, S. A. Boyko, J. R. Butterton, J. A. Stoebner, S. M. Payne, and S. B. Calderwoo...

The prevalence of pleuropneumonialike organisms (PPLO) has been summarized by Morton et al. (1952). Their incidence and significance in infections of the genitourinary tract have been discussed by Ruiter and Wentholt (1953a) and Edward (1952). Subsequent reports of the finding of these organisms in infections of man (Slingerland and Morgan, 1952; Ruiter and Wentholt, 1953b) and of poultry (Mark...

Mammalian erythrocytes normally contain high concentrations of the tripeptide GSH.* The major role of G S H in these cells is t o protect against oxidative damage, the GSH-GSSG coupleactingasaredox bufferingsystem. Sheepexhibit two distinct types of inherited erythrocyte GSH deficiency. One type is associated with a diminished activity of y-glutamyl-cysteine synthetase, the first enzyme of GSH ...

The effect of oral administration of thiocyanate, tellurite and hydroxyurea on haematological profile in albino rats was investigated in this study. Rats were divided into four groups. Control group received distilled water while the three experimental groups were administered with each of the antisickling drug daily at their therapeutic dose for a period of 28 days. All the drugs significantly...

Stenotrophomonas maltophilia is an aerobic, non-fermentative Gram-negative bacterium widespread in the environment. S. maltophilia Sm777 exhibits innate resistance to multiple antimicrobial agents. Furthermore, this bacterium tolerates high levels (0.1 to 50 mM) of various toxic metals, such as Cd, Pb, Co, Zn, Hg, Ag, selenite, tellurite and uranyl. S. maltophilia Sm777 was able to grow in the ...

Cell-free extracts of Thermus thermophilus HB8 catalyze the in vitro, NADH-dependent reduction of potassium tellurite (K2TeO3). Three different protein fractions with tellurite-reducing activities were identified. Two exhibited high molecular weight and were composed of at least two different polypeptides. The protein in the third fraction was purified to homogeneity and had a single polypeptid...