In vitro RNA synthesis by tobacco mosaic virus and cowpea chlorotic mottle virus replicase were inhibited by cordycepin triphosphate. Inhibition could be overcome with higher concentrations of ATP in assay mixtures but not with UTP. Products synthesized in vitro by tobacco mosaic virus RNA replicase in the presence of inhibitor revealed replicative form but not replicative intermediate RNAs. Th...

alfalfa mosaic virus (almv) is one of the most conspicuous alfalfa viruses in the world. in order to identify the distribution, host range and molecular characterization of alfalfa mosaic virus in kerman province, 514 alfalfa samples showing mosaic, calico, mottle and malformation symptoms as well as 66 potato samples showing calico symptoms were collected from alfalfa and potato fields in kerm...

The surprise was great in the early 1970s when it was shown that valine could be covalently attached to the 3' terminus of the genomic RNA from turnip yellow mosaic virus (TYMV) by valyl-tRNA synthetase (1) and, soon after, that tyrosyland histidyl-tRNA synthetases can aminoacylate the RNAs from brome mosaic virus (BMV) and tobacco mosaic virus (2, 3). The surprise was even greater when it was ...

Background and Aims: Certification programs of plant propagating materials rely on faster, cheaper and more importantly sensitive and reliable methods for detection of systemic pathogens as indicated in national and/or international health standards of plant propagating materials. Reverse transcription-polymerase chain reaction (RT-PCR) has been documented as an alternative assay for certific...