Amanda Gonçalves ([email protected]) Ingrid P Ewald ([email protected]) Marina Sapienza ([email protected]) Manuela Pinheiro ([email protected]) Ana Peixoto ([email protected]) Amanda F Nobrega ([email protected]) Dirce M Carraro ([email protected]) Manuel R Teixeira ([email protected]) Patricia Ashton-Prolla ([email protected]...

Author affiliations: School of Medicine of the Federal University of Ceará, Fortaleza, Brazil (D.C.S. Monteiro, A.Q. Sousa, R.M. Fontes, C.C. Praciano, M.S. Frutuoso, L.C. Matos, M.J. Teixeira, M.M.L. Pompeu); School of Medicine of the University of Fortaleza, Fortaleza (D.M. Lima); Unichristus School of Medicine, Fortaleza (M.S. Frutuoso); University of Virginia School of Medicine, Charlottesv...

BENCHMARKS Conditional gene expression using the Tet system is well established in eukaryotes (1). The tetracycline-controlled transactivator (tTA) binds to P tet-1 in the absence of an effector and stimulates transcription of the successive target gene (2). Effector tet-racyclines abolish activation by tTA (Tet-Off). The reverse transactivator rtTA2 S-M2 contains a mutated tetR al-lele and act...

steady state natural convection of al2o3-water nanofluid inside a square cavity filled with a porous medium is investigated numerically. the temperatures of the two side walls of the cavity are maintained at th and tc, where tc has been considered as the reference condition. the top and the bottom horizontal walls have been considered to be insulated i.e., non-conducting and impermeable to mass...

the present study describes the encapsulation of protocorm of cymbidium bicolor lindl. from 60 days-old in seed cultures for short- term conservation and propagation. various concentrations and combinations of gelling matrix (sodium alginate) and complexing agents (calcium chloride) were tested to prepare uniform beads. the ideal beads were ob- tained through a combination of 3% sodium alginate...

Preliminary reports have demonstrated that technetium (Tc)-labeled cyclic [Cys, D-Phe]a-MSH3–13 (CCMSH) exhibits high tumor uptake and retention values in a murine melanoma mouse model. In this report, the tumor targeting mechanism of Tc-CCMSH was studied and compared with four other radiolabeled a-melanocyte stimulating hormone (a-MSH) peptide analogues: I-(Tyr)-[Nle, D-Phe]a-MSH [I(Tyr)-NDP];...

A full-length DNA copy of cymbidium ringspot virus RNA was cloned downstream of a phage T7 promoter. In vitro transcripts had no extra nucleotides at the 3' terminus, and a 5' end likely to be precisely as in genomic RNA. Transcripts were infective when inoculated into test plants. Northern blots from inoculated plants revealed the presence of genomic and subgenomic RNAs, but not of satellite R...

Nicotiana clevelandii plants were inoculated with cymbidium ringspot tombusvirus RNA synthesized in vitro, after which further passages were made by sap inoculation. During the third passage, low Mr RNA species appeared which had the characteristics of deletion mutants of genomic RNA. Sequence analysis of several of these defective interfering RNAs suggested a possible evolution of smaller from...

in the present study, variation among different bacterial strains was observed in the plaques of tobacco chewers (tc) as compared to those from normal persons (non tobacco chewers) (ntc). bacterial strain j1 (dominant in the plaques of ntc) did not initiate the process of adhesion (biofilm formation) until it appeared to have reached a required population density necessary for the production of...