1. Rat thyroid lobes were incubated for various periods of time in Krebs-Ringer bicarbonate containing [3H]leucine and either [1-14C]galactose or [1-14C]mannose. Radioactivity in soluble proteins was determined after their separation by sucrosegradient centrifugation. 2. The time-course of incorporation of label from [14C]mannose into soluble thyroid proteins was parallel to that observed for [...

This paper describes a method for quantitative extraction of nucleic acids from cultured pre-implantation mouse embryos in a form suitable for electrophoresis, and its application to the analysis of RNA synthesized during early mouse development. The nucleic acids synthesized by early mouse embryos have been identified by the use of RNase, DNase and mild alkaline hydrolysis. No obvious differen...

Micrometer-sized Janus particles of many kinds can be formed using droplet microfluidics, but in existing methods, the microfluidic templating is strongly coupled to the material synthesis, since droplet solidification occurs through rapid polymerization right after droplet formation. This circumstance limits independent control of the material properties and the morphology of the resultant par...

1. The mode of uptake ofthe precursors ofmilk fat by the mammary gland of the lactating goat has been examined by infusing radioactive fatty acids, glycerol or doubly labelled triglycerides into the mammary artery or jugular vein of animals surgically prepared to permit samples of arterial and venous blood to be withdrawn without disturbance to the animal. 2. Acetate was taken up by the mammary...

Using the first principle methods, we proposed a series of three-chain boron cages B6n+14 (n = 1-12) which are mainly built by fusing three boron semi-double-rings. Their simple geometric structures (approximate D3 or C3 symmetry) facilitate their bottom-up syntheses from the hexagonal B7 and the double-chain boron clusters, such as B2, B4, B6, B8H2, B10H2, B12H2, and the double ring B20. The s...

This paper seeks to extend our knowledge about RNA synthesis in early embryogenesis to the domestic fowl, Gallus domesticus. Using this species for research, apart from increasing our knowledge of higher vertebrate embryology, has certain advantages such as rapid uptake of isotopic precursors and ease of microdissection in culture. The following results are presented: (1) The cell number in the...

Protein synthesis before and after fertilization in Zlyanassa obsoleta has been investigated. Unfertilized eggs do incorporate significant amounts of labelled amino acids into protein. However, fertilized eggs undergo an increase both in uptake and incorporation of labelled ammo acids into protein within 15 minutes of fertilization. Post-fertilization protein synthesis is sensitive to but not c...