نتایج جستجو برای: sybr green

تعداد نتایج: 140135  

2009
Selcuk M. Ozbek Ahmet Ozbek Aziz S. Erdogan

OBJECTIVE The aims of this study were to investigate the presence of Enterococcus faecalis in primary endodontic infections and failed endodontic treatments using real-time PCR and to determine the statistical importance of the presence of E. faecalis in a Turkish population with endodontic infections. MATERIAL AND METHODS E. faecalis was investigated from 79 microbial samples collected from ...

Journal: :Journal of microbiological methods 2003
Michał Mikula Artur Dzwonek Katarzyna Jagusztyn-Krynicka Jerzy Ostrowski

Accurate diagnosis of Helicobacter pylori infection is important in both clinical practice and clinical research. Molecular methods are highly specific and sensitive, and various PCR-based tests have been developed to detect H. pylori in gastric biopsy specimens. We optimized a sensitive and specific quantitative SYBR Green I real-time PCR assay for detection of H. pylori based on amplification...

Journal: :Genetics and molecular research : GMR 2015
G J Chang H M Seyfert X Z Shen

In the mammalian genome, approximately 50% of all genes are controlled by promoters with high GC contents. Analyzing the epigenetic mechanisms regulating their expression is difficult. Hence, we examined a method for stable quantification of such GC-rich DNA sequences. Quantification of DNA during real-time PCR is often based on reagent kits containing the fluorescent dye SYBR Green. However, t...

Journal: :The Journal of antimicrobial chemotherapy 2011
Tomoko Arai Isao Kimata Yukio Kitade Kentaro Nakamoto Masaharu Tokoro

OBJECTIVES The aims of this study were to provide a cost-effective and valuable method for evaluating drug efficacy against Cryptosporidium parvum using a quantitative SYBR Green real-time PCR (qPCR) and to assess the efficacy of adenosine analogues as drug templates. METHODS C. parvum HNJ-1 strain growing in human ileocaecal adenocarcinoma cells was employed as an in vitro culture system. To...

Journal: :Applied and environmental microbiology 2006
Willm Martens-Habbena Henrik Sass

The determination of cell numbers or biomass in laboratory cultures or environmental samples is usually based on turbidity measurements, viable counts, biochemical determinations (e.g., protein and lipid measurements), microscopic counting, or recently, flow cytometric analysis. In the present study, we developed a novel procedure for the sensitive quantification of microbial cells in cultures ...

Journal: :Journal of clinical microbiology 2004
James F Papin Wolfgang Vahrson Dirk P Dittmer

Real-time quantitative PCR is used routinely for the high-throughput diagnosis of viral pathogens, such as West Nile virus (WNV). Rapidly evolving RNA viruses present a challenge for diagnosis because they accumulate mutations that may render them undetectable. To explore the effect of sequence variations on assay performance, we generated every possible single point mutation within the target ...

2012
Xiaoyun Chen Xiaofu Wang Nuo Jin Yu Zhou Sainan Huang Qingmei Miao Qing Zhu Junfeng Xu

Genetically modified (GM) rice KMD1, TT51-1, and KF6 are three of the most well known transgenic Bt rice lines in China. A rapid and sensitive molecular assay for risk assessment of GM rice is needed. Polymerase chain reaction (PCR), currently the most common method for detecting genetically modified organisms, requires temperature cycling and relatively complex procedures. Here we developed a ...

Journal: :Cytometry. Part A : the journal of the International Society for Analytical Cytology 2007
Patricia Assunção Hazel M Davey Ruben S Rosales Nuno T Antunes Christian de la Fe Ana S Ramirez Carlos M Ruiz de Galarreta Jose B Poveda

The detection of mycoplasma in milk can be performed by either culture techniques or polymerase chain reaction (PCR) based methods. Although PCR can reduce the average diagnostic time to 5 h in comparison with the several days for the isolation of the agent, there is still a need to develop methods, which could give earlier results. For this purpose, we tested the ability of flow cytometry (FC)...

2017
Ma. Carmen E. Delgado-Gardea Patricia Tamez-Guerra Ricardo Gomez-Flores Aurora Mendieta-Mendoza Francisco Javier Zavala-Díaz de la Serna Juan Francisco Contreras-Cordero Gilberto Erosa-de la Vega María Concepción Pérez-Recoder Blanca Sánchez-Ramírez Carmen González-Horta Rocío Infante-Ramírez

In areas lacking potable water treatment, drinking contaminated water may represent a public health threat. In addition to enteropathogenic bacteria and parasites, fecal contamination in water environments is associated with the transmission of enteric viruses and other causal agents of infectious disease. Rotavirus and norovirus are the main enteric viral agents responsible for diarrheic outbr...

2012
Riitta Paakkanen Hanna Vauhkonen Katja T. Eronen Asko Järvinen Mikko Seppänen Marja-Liisa Lokki

Low protein levels and copy number variation (CNV) of the fourth component of human complement (C4A and C4B) have been associated with various diseases. High-throughput methods for analysing C4 CNV are available, but they commonly do not detect the most common C4A mutation, a silencing CT insertion (CTins) leading to low protein levels. We developed a SYBR® Green labelled real-time quantitative...

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