Porous organic materials have received increasing attention due to their potential applications, such as gas storage, separation, and catalysis. In this work we present a series of aromatic, polyazide-containing building blocks that enable the formation new class amorphous porous materials. The azide precursors are obtained in moderate good yields following an easy synthesis procedure. By therm...

The course of reaction of aryl and heteroaryl sulfonamides with diphenylcarbonate (DPC) and 4-dimethylaminopyridine (DMAP) was found to depend on the pKa of the sulfonamide used. Aryl sulfonamides with pKa approximately 10 gave 4-dimethylamino-pyridinium arylsulfonyl-carbamoylides, while the more acidic heteroaryl sulfonamides (pKa approximately 8) furnished 4-dimethylaminopyridinium heteroaryl...

The title compound, C17H27NO2S, exhibits a distorted geometry of the aromatic ring with elongated bonds at the ipso-C atom. The S atom deviates from the aromatic ring plane by 0.393 (4) Å. Similar to this, the adjacent isopropyl groups are bent out of the aromatic ring plane by -0.125 (4) and -0.154 (4) Å. Even the distant isopropyl group in para-position to the sulfonyl moiety shows a slight d...

Highly purified iron superoxide dismutase was obtained from Escherichia coli B using a modification of the procedure of Yost and Jridovich (Yost, F. J., Jr., and Fridovich, I. (1973) J. Biol. Chem. 248, 4905-4908). The protein contained 1.8 +/- 0.2 atoms of iron per 38,700 g of protein. We have found that cyanide does not bind to the Fe3+ ion of iron dismutase but fluoride and azide have modera...

Grape is important crop with high nutritional value, however there is a great need for improving the quality of the crop. Cultures were maintained in vitro on MS medium supplemented with 2.5 mg/L BAP+ TDZ 3.0 mg/ L. The effect of different doses of gamma irradiation as well as chemical mutagenesis by Sodium azide was studied. It was noted that doses up to 5 Gy helped to increase plant height fr...

We report an assay for determining the number of fluorophores conjugated to single plasmid DNA molecules and apply this to compare the efficiency of fluorophore coupling strategies for covalent DNA labelling. We compare a copper-catalyzed azide-alkyne cycloaddition reaction, amine to N-hydroxysuccinimidyl ester coupling reaction and strain-promoted azide-alkyne cycloaddition reaction for fluore...

Synthesis of organic bilayers on silicon was realized by a combination surface functionalization under ultrahigh vacuum (UHV) conditions and solution-based click chemistry. The (001) prepared with high degree perfection in UHV functionalized via chemoselective adsorption ethynyl cyclopropyl cyclooctyne from the gas phase. A second layer then coupled acetonitrile copper-catalyzed alkyne–azide re...

Azide binding to the blue copper oxidases laccase and ascorbate oxidase (AO) was investigated by electron paramagnetic resonance (EPR) and pulsed electron-nuclear double resonance (ENDOR) spectroscopies. As the laccase : azide molar ratio decreases from 1:1 to 1:7, the intensity of the type 2 (T2) Cu(II) EPR signal decreases and a signal at g approximately 1.9 appears. Temperature and microwave...

By accurately measuring the difference infrared spectrum of Cot-equilibrated bovine carbonic anhydrase against the ethoxzolamideor azide-inhibited enzyme, we have been able to obtain the absorption maximum at 2341 cm-l that is due to the asymmetric stretching of the CO2 molecule loosely bound to a hydrophobic surface at the active site of the enzyme. COz dissolved in water absorbs at 2343.5 cm-...

The aim of the current study was to investigate the effect of mitochondrial division inhibitor 1 (Mdivi‑1) in sodium azide‑induced cell death in H9c2 cardiac muscle cells. Mdivi‑1 is a key inhibitor of the mitochondrial division protein dynamin‑related protein 1 (Drp1). Mdivi‑1 was added to H9c2 cells for 3 h, after which, the cells were treated with sodium azide for 24 h. Cell viability was me...