We investigated the influence of organic substrates and phosphate concentration on the rates of dissimilatory microbial sulfate reduction and the 34S/32S isotopic fractionation produced by several Desulfovibrio species. Our experiments corroborate the previously reported species-specific correlation between sulfur isotope fractionation and cell-specific sulfate reduction rates. We also identify...

erythroblastosis virus was purified from culture fluids by high-speed clarification, concentration through a 5% sucrose barrier, and centrifugation through a discontinuous sucrose gradient. Viral RNA was extracted by treatment of purified virions with 0.5% sodium dodecyl sulfate or phenolisodium dodecyl sulfate. RNA profiles from virus of 24-hr culture fluids were heterodisperse and included la...

This work reports the chemical and light extinction characteristics of the atmospheric particles collected from January to November 2014 in suburban Nanjing. Size-segregated measurement results showed that more than 80% of the major aerosol components were concentrated in PM2.5. The concentration of PM2.5 was highest in winter and lowest in autumn. Specifically, K+ concentration peaked in late ...

Background and Objectives: In the original in vitro method for evaluation of iron bioavailability using caco-2 cell model, ferritin formation by cells is considered as a functional indicator of both absorption and utilization of iron. However, as iron is measured in the tested material and then ferritin concentration is measured in caco-2 cell lysate, it is almost impossible to have a concrete ...

Reconstitution of bacteriorhodopsin into vesicles has been studied using individual and defined mixtures of fractionated Halobacterium halobium polar lipids. The lipids varied greatly in their ability to form vesicles that translocated protons and showed stimulation by valinomycin. Thus, phosphatidylglycerol phosphate gave mainly lipid-protein aggregates, while glycolipid sulfate gave mainly fu...

Dermatan sulfate is a naturally occurring antithrombotic glycosaminoglycan. The antithrombin activity of several dermatan sulfate preparations has been measured in whole human plasma and found to be -55% of that in purified systems. Kinetic studies under pseudo-first-order conditions indicated that the reduction in antithrombin activity of dermatan sulfate in plasma compared with that in buffer...

Kinetics of the oxidation of tris(2,2′-bipyridine)iron(II) sulfate by ceric sulfate was spectrophotometrically studied in an aqueous sulfuric acid medium. Different methods, including isolation, integration and half-life, were employed to determine the reaction order. The redox reaction was found to be first-order with respect to the reductant, tris(2,2′-bipyridine)iron(II) sulfate, and the oxi...

A physico-chemical model for the accumulation of sulfur species in raindrops is developed in which account is taken of the mass transfer of SO1, 0,, NH, and CO1 into ideal raindrops containing the catalyst Fe(II1). The sulfur accumulation was calculated for the droplets as a function of fall distance. The model predicts the formation of sulfate due to the oxidation of dissolved SO2 by 0, and ca...

Ten strains of Pseudomonas aeruginosa were disrupted and centrifuged. The supernatant fluids from centrifugation at 105,000 X g contained enzymes inactivating kanamycin, neomycin, and streptomycin in the presence of adenosine triphosphate. Kanamycin-inactivating enzyme was precipitated with ammonium sulfate at 66% of saturated concentration, and the inactivated kanamycin was shown to be kanamyc...

Metabolically stable anaerobic enrichment cultures have been obtained from sediment samples contaminated with chlorophenolic compounds. Enrichment was carried out with esculin, esculetin, naringin, naringenin, fraxin, quercetin, and acetate in media with two sulfate concentrations. These cultures were used to examine the O-demethylation of 4,5,6-trichloroguaiacol and the dechlorination of 3,4,5...