HIGHLIGHTS DSPRED method based on machine learning algorithms to predict 3-state secondary structure elements. Comparative results for prediction. High accuracy of 82.36% SCOPe (Structural Classification Proteins - extended) structural classes.

tissue plasminogen activator (tpa) is a serine protease, which is composed of five distinct structural domains with 17 disulfide bonds, representing a model of high-disulfide proteins in human body. one of the most important limitations for high yield heterologous protein production in escherichia coli (e. coli) is the expression of complex proteins with multiple disulfide bridges. in this stud...