Actinomycetes were isolated from soybean rhizosphere soil collected as two field sites in Brazil. All the isolates were identified as Streptomyces species and were screened for streptomycin production and the presence of two genes, strA and strB1, known to be involved in streptomycin biosynthesis in Streptomyces griseus. Antibiotic resistance profiles were determined for 53 isolates from cultiv...

The streptomycin counter-selection system is a useful tool for constructing unmarked in-frame gene deletions, which is a fundamental approach to study bacteria and their pathogenicity at the molecular level. A prerequisite for this system is acquiring a streptomycin-resistant strain due to rpsL mutations, which encodes the ribosomal protein S12. However, in this study no streptomycin resistance...

INTRODUCTION Aminoglycosides like streptomycin are well-known for binding at specific regions of ribosome RNA and then acting as translation inhibitors. Nowadays, several pathogens have been detected to acquire an undefined strategy involving mutation at non structural ribosome genes like those acting as RNA methylases. rsmG is one of those genes which encodes an AdoMet-dependent methyltransfer...

interaction of two complementary growth-factor dependent mutants, i.e., Tzp lemen ta ry auxotrophs, of Streptomyces coeticolor to form grovvth-factor independent progeny, i.e., prototrophs, was interpreted as gene recombination by SERMO~TI and SPADA-SERMONTI (1955) and by BRAENDLE and SZ+BALSKI (1959yi but as heterokaryosis by BRADLEY (1957). The former workers concluded that gene recombination...

Shortly after streptomycin was introduced in the treatment of tuberculosis, two major obstacles to its value as a therapeutic agent became apparent. These were, vestibular damage and development of bacterial resistance. With the reduction in the daily dosage from 2 gm. and higher, to 1 gm. and later to 0.5 gm., serious vestibular damage has been eliminated. However, development of bacterial res...

H.j CHAKRAVARTI (School of Tropical Medicine, Calcutta) Streptomycin, when parenterally administered, is partially excreted and concentrated in the bile (Zintel ct ai, 1945), and in this way it can be effectively used in any infection of the biliary tract by streptomycin-sensitive organisms. In the transient jaundice of infective hepatitis, a viral infection, streptomycin is of no use. But in t...

Electrostatic interaction between streptomycin and mercaptoacetic acid modified gold nanoparticles can be used for a facile and reliable probe for streptomycin with high sensitivity and selectivity.

RECENTLY, streptomycin dependence and resistance in bacteria have come to be viewed as accommodations requiring or permitting certain alterations by streptomycin of the expression of other genetic traits carried by the organism (e.g., GORINI 1964). For future evaluation of this viewpoint, it would be useful to have information about the relation of genetic factors governing dependence, resistan...