Group B streptococci (GBS) express various surface antigens designated c, R, and X antigens. A new R-like surface protein from Streptococcus agalactiae strain Compton R has been identified by using a polyclonal antiserum raised against the R protein fraction of this strain to screen a lambda Zap library. DNA sequence analysis of positive clones allowed the prediction of the primary structure of...

The function of the nuclear envelope in regulating the cellular distribution of proteins was studied by experimentally altering nuclear permeability and determining the effect of the procedure on the incorporation of exogenous and endogenous polypeptides into the nucleoplasm. Using fine glass needles, nuclear envelopes were disrupted by puncturing oocytes in that region of the animal pole occup...

Electron microscopy is a technology for examining the extremely fine detail or ultrastructure of biological specimens. This methodology opened a totally new vista to the human eyewhenbiological ultrastructurewas revealed as a dynamic and architecturally complex arrangement of macromolecules under the direction of genetic units termed genes. Complex subcomponents of the cell, termed organelles, ...

Various dilutions of anti-nerve growth factor serum from rabbits or horses immunized with purified nerve growth factor were added to rapidly growing, 48-hr monolayer cultures of a human glioblastoma (C356) and of an H. Ep. 2 cell line. Cytotoxic effects, beginning 2 hr later and usually progressing to complete cell death at 17 hr, were studied in vitro in stained coverslip preparations and by e...

Various dilutions of anti-nerve growth factor serum from rabbits or horses immunized with purified nerve growth factor were added to rapidly growing, 48-hr monolayer cultures of a human glioblastoma (C356) and of an H. Ep. 2 cell line. Cytotoxic effects, beginning 2 hr later and usually progressing to complete cell death at 17 hr, were studied in vitro in stained coverslip preparations and by e...

Immunoglobulin from goat antiserum directed against purified surface membranes from transformed BHK21/C13 cells (anti-M) has been shown to cause both control and transformed hamster cells to round and detach from the substrate (see accompanying paper). This paper documents the effects of the antiserum on the cytoskeletal organization and cell surface morphology of control BHK21/C13 cells examin...

Electron microscopy analyses of Iguana iguana blood preparations revealed the presence of mitochondria within erythrocytes with well-structured cristae. Fluorescence microscopy analyses upon incubation with phalloidin-FITC, Hoechst 33342 and mitochondrial transmembrane potential (Δψm)-sensitive probe MitoTracker Red indicated that mitochondria i) widely occur in erythrocytes, ii) are polarized,...

Electron microscopy uses high-energy elec tron beams to probe the structure and chem istry of both inorganic and biological materi als. The short wavelength (A) of electrons permits higher resolution images than light microscopes. This was a major motive in the early development of the transmission elec tron microscope (TEM) by Knoll and Ruska (1932) in Berlin, and atomic resolution is now rout...

THE well-deserved popularity of this book and the rapidly expanding nature of the subject has justified a third edition only four years after the second edition had been introduced. As in previous editions, each chapter consists of an introduction to the subject, practical details of the analytical methods and a comprehensive clinical interpretation. A completely new chapter on enzymes has been...