نتایج جستجو برای: strain homogeneity
تعداد نتایج: 237230 فیلتر نتایج به سال:
A new deoxyribonuclease, PaeExo IX, has been purified to electrophoretic homogeneity from extracts of Pseudomonas aeruginosa strain PAO. This enzyme, which is active in the presence of EDTA, is equally efficient in hydrolyzing native and heart-denatured DNA to acid-s-luble products. The enzyme is partially or totally inhibited by the presence of several divalent cations. The active protein has ...
NAD+-dependent formate dehydrogenase was screened in various bacterial strains. Facultative methanol-utilizing bacteria isolated from soil samples, acclimated to a medium containing methanol and formate at pH 9.5, were classified as members of the genus Moraxella. From a crude extract of Moraxella sp. strain C-1, formate dehydrogenase was purified to homogeneity, as judged by disc gel electroph...
Here we report on translocation of short poly-arginines across the MOMP porin, the major outer membrane protein in the cell wall of Campylobacter jejuni. MOMP was purified to homogeneity from a pathogenic strain of C. jejuni. Its reconstitution in lipid membranes and measuring the ion-current revealed two main distinct populations of protein channels which we interpreted as mono and trimers. Ad...
In this paper we investigate the effect of microstructure irregularities and applied strain on backward Brillouin scattering by comparing two photonic crystal fibers drawn with different parameters in order to minimize diameter and microstructure fluctuations. We fully characterize their Brillouin properties including the gain spectrum and the critical power. Using Brillouin echoes distributed ...
Superoxide dismutase has been purified to homogeneity from aerobically grown Thiobacillus denitrijicarzs strain “RT”. It has a molecular weight of 43,000, is composed of two identical subunits which are not covalently bound, and contains 1.35 atom of iron per molecule. Absorption spectra and amino acid analysis are similar to those of other Fe-superoxide dismutases from bacteria. Aerobically an...
A three-dimensional finite element model of the joint has been developed. The model has been used to predict the stress field of the laminate in the joint area, assuming homogeneity and linear behavior of the material. The modeled geometry is a T-bolt that joins a coupon of glass fiber–epoxy and a block of steel. The results obtained from the model have been verified experimentally using both e...
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