Pseudomonas putida KT2440 is an emerging biomanufacturing host amenable for use with renewable carbon streams including aromatics such as para-coumarate. We used a pooled transposon library disrupting nearly all (4,778) non-essential genes to characterize this microbe under common stirred-tank bioreactor parameters quantitative fitness assays. Assessing differential values by monitoring changes...

The main bottleneck in fermentation technology is scaling up procedure of industrial enzymes according to the biological characteristics organism. current study describes production kinetics cellulases stirred tank reactor by using mutant and wild strains T. dupontii. span both bioreactor was examined. It found strain dupontii time required for optimum reduced 48h as compared 72h strain. kineti...

Insufficient mixing in large-scale bioreactors provokes gradient zones of substrate, dissolved oxygen (DO), pH, and other parameters. E. coli responds to a high glucose, low feeding zone with the accumulation mixed acid fermentation products, especially formate, but also synthesis non-canonical amino acids, such as norvaline, norleucine ?-methylnorleucine. These acids can be mis-incorporated in...

-Feeding continuously operated stirred tank rectors with reactants and homogeneous catalysts subject to inactivation will usually lead to a limited substrate conversion with increasing space time. However, it is shown that a maximum of conversion may be observed under certain circumstances. Guided by experimental evidence, the theoretical background is discussed for identifying reaction systems...

This paper presents control of the nonlinear system represented by a Continuous Stirred Tank Reactor (CSTR) using MATLAB’s Graphical User Interface (GUI). This simulation provides computation for various values of the input variables, which made this program universal for the wide range of the users without theoretical knowledge about modeling, simulation, and control. (

The observer design problem for a class of cell population balance models, describing the time evolution mass density distribution function and substrate concentration in continuous-stirred tank bioreactor with irregular discrete-time measurements is considered. model consists partial integro-differential equation coupled an ordinary differential equation. Using theory impulsive systems suffici...

Lactococcus lactis is highly efficient probiotics microorganism with wide range of benefits on human health. This study was conducted to design and establish industrial platform for high cell density cultivation of this novel probiotic strain, L. lactis (WICC-B25). During bioreactor cultivation of lactic acid bacteria in industrial scale, there are two main problems during process namely: low c...