Ccr4 is a major cytoplasmic deadenylase involved in mRNA poly(A) tail shortening in Saccharomyces cerevisiae. We have previously shown that Ccr4 negatively regulates expression of LRG1 mRNA encoding a GTPase-activating protein for the small GTPase Rho1, a component of cell wall integrity pathway, and deletion of LRG1 suppresses the temperature-sensitive growth defect of the ccr4Δ mutant. We hav...

A cloned esterase and several cytoplasmic proteins which lack a classical cleavable signal-peptide were secreted during late stationary phase in B. subtilis. Several lines of evidence indicate that secretion of enolase, SodA, and Est55 is not due to cell lysis. The extent of possible release of these proteins mediated by membrane vesicles into the medium was also found to be minimal. We have id...

In this work, novel atom-type-based topological indices, named AT indices, were presented as descriptors to encode structural information of a molecule at the atomic level. The descriptors were successfully used for simultaneous quantitative structure-retention relationship (QSRR) modeling of saturated alcohols on different stationary phases (SE-30, OV-3, OV-7, OV-11, OV-17 and OV-25). At first...

This contribution summarizes methods to compare bonded stationary phases for the compound-class HPLC separations of unsaturated polycyclic hydrocarbons and polar compounds. These strategies are based on the physical chemistry behavior of the functional group of the bonded stationary phase, behavior of the unsaturated polycyclic hydrocarbons under isocratic and gradient elution, regression analy...

Using the method of prolongation we generate new stationary solutions from a recently obtained simple particular solution for relativistic transverse flow with cylindrical symmetry in 1+3 dimension. This is an extension of the longitudinal Bjorken flow ansatz and can be applied to situations during a first order phase transition in a fast expanding system. The prolongated solution allows us to ...

The present study was designed to develop a simple, validated liquid chromatographic method for the analysis of azithromycin in bulk and pharmaceutical dosage forms using ultraviolet detector. The best stationary phase was determined as C18 column, 5 µm, 250 mm × 4.6 mm. Mobile phase was optimized to obtain a fast and selective separation of the drug. Flow rate was 1.5 mL/min, Wavelength was se...