The kinesin motor protein family members move along microtubules with characteristic polarity. Chimeric motors containing the stalk and neck of the minus-end-directed motor, Ncd, fused to the motor domain of plus-end-directed kinesin were analyzed. The Ncd stalk and neck reversed kinesin motor polarity, but mutation of the Ncd neck reverted the chimeric motor to plus-end movement. Thus, residue...

Visit our website at: http://pituitary.mgh.harvard.edu On magnetic resonance imaging, some patients with clinical neuroendocrine disorders have thickened pituitary stalks, the anatomical structure which connects the hypothalamus to the pituitary (Figure 1). These disorders can be classified as neoplastic, inflammatory, or infectious in origin, although there are rare forms of congenital malform...

We report the observation of a phase of phospholipid that contains a structure similar to the commonly postulated interbilayer state that is crucial to membrane fusion. The widely accepted model for membrane fusion suggests that there is an intermediate state in which the two contacting monolayers become continuous via an hourglass-shaped structure called a stalk. Many efforts have been made to...

We characterized human monoclonal antibodies (MAbs) cloned from influenza virus-infected patients and from influenza vaccine recipients by complement-dependent lysis (CDL) assay. Most MAbs active in CDL were neutralizing, but not all neutralizing MAbs can mediate CDL. Two of the three stalk-specific neutralizing MAbs tested were able to mediate CDL and were more cross-reactive to temporally dis...

We describe a case of body stalk anomaly which was diagnosed at 10 weeks of gestation on a dating scan. The fetus was visualized within the exocelomic cavity outside an apparently intact amniotic membrane. The fetus displayed multiple anomalies characteristic of body stalk syndrome including a skull and brain defect, abdominal wall defect, kyphoscoliosis and gross deformities of the lower limbs...

stalk being 10–13 residues shorter than that of the 45-residue CD8␣ stalk (Kern et al., 1998). The presence of numerous proline residues plus O-glycans in both stalks indicate that they assume an extended conformation (Classon et al., 1992). CD8 glycan adducts change dur

The energetics of a fusion pathway is considered, starting from the contact site where two apposed membranes each locally protrude (as "nipples") toward each other. The equilibrium distance between the tips of the two nipples is determined by a balance of physical forces: repulsion caused by hydration and attraction generated by fusion proteins. The energy to create the initial stalk, caused by...

We report the cDNA sequence of the squid kinesin heavy chain and compared the predicted amino acid sequence with that of the Drosophila heavy chain as reported by Yang, J.T., Laymon, R.A., and Goldstein, L.S. B. (1989) Cell 56, 879-889). We compared the two kinesin sequences with regard to the predicted physicochemical parameters of hydrophobicity, charge, and propensities of the secondary conf...

The overall objective of this investigation is to develop a technology to employ biomass as substitute fuel for coal to help reduce NOx.. It was achieved by governing NOx formation kinetics through in-furnace air and fuel staged co-combustion technology. An extensive series of experimentation was carried out in a 20 kW down fired combustor to evaluate the effect of co-firing of Shea meal, Cotto...

In this paper, we introduce the concept of hierarchy-based fault-local stabilization and a novel self-healing/fault-containment technique and apply them in Stalk. Stalk is an algorithm for tracking in sensor networks that maintains a data structure on top of an underlying hierarchical partitioning of the network. Starting from an arbitrarily corrupted state, Stalk satisfies its specification wi...