In order to establish a method usable in the field for diagnosis of pre-clinical vitamin A deficiency, conjunctival impression cytology with transfer (CICT) was used in 200 normal slum children aged 6-120 months in Chandigarh, India. Conjunctival impressions taken on cellulose acetate paper were transferred to glass slides which were fixed in ethanol and stained with alcian blue and carbol fuch...

We studied the laminar organization of 3T3 fibroblast cells growing on glass slides by use of total internal reflection illumination to excite fluorescence emission (TIRF) from labeled molecules and stained cellular compartments that are very close to the cell-substrate contact region. Mitochondria, distant from the contact regions and stained with the water-soluble cationic dye, dil-C3-(3), fl...

The present article is devoted to the analysis of structural elements of a room prepared for comfort living. A new variant for minimizing the space in a room for comfort living is shown on the example of a studioapartment in two levels on an attic floor. An overview of the apartment is shown, as well as layout plans of floors with furniture. Favorable transitory elements between zones in the ro...

The surface of a separate Dictyostelium discoideum cell exposed to fluorescent antiserum is initially uniformly stained. When the cell moves, labelled material is left on the glass over the area occupied by the cell while in the antiserum. The surface of new pseudopods is fluorescent. The bulk of the label soon accumulates on the rear of the cell and is towed along behind it. The rest of the su...

The article is devoted to the art project “Jesus of Nazareth King Jews” / I. N. R. as an example modern religious art. was exhibited at Pilgrimage Museum in Germany 2020 and presents environment, where sculpture, painting, graphics stained glass windows dedicated events Gospel history with emphasis on Passion Christ are combined space several halls. author turned traditions modernist art, inclu...

Isolation of proteins that specifically interact with a given RNA or RNA regulation element is essential for studies on the molecular mechanisms of gene expression. Here, a novel method for direct isolation of such interacting proteins is described. It uses an affinity medium that consists of an interacting RNA with an artificially added 'tail', which is annealed to one end of a DNA 'arm', the ...

Microleakage is related to recurrent decay, inflammation of vital pulps, and reinfection of previously treated root canals. The purpose of this investigation was to compare the abilities of new adhesive cements and conventional nonadhesive controls to prevent microleakage under stainless steel crowns on primary anterior teeth. Standardized preparations were made, and stainless steel crowns were...

BACKGROUND Obtaining high-quality images of cellular structures via immunofluorescence staining is critical for cellular localization studies. Often, these studies cannot be performed in parallel with certain oncology, virology, pharmacokinetic, and drug absorption studies due to model system technicalities requiring the cells to be cultured on porous membranes rather than glass or plastic. MAT...