The distribution and subcellular localization of microtubules in rat brain cerebellum was analyzed by immunohistochemistry with antibodies prepared against 3 synthetic peptides corresponding to the C-terminal region of beta-tubulin. The peptides used correspond to amino acid positions 416-425 (peptide 1), 416-431 (peptide 2), and 426-445 (peptide 4). The antibodies thus obtained displayed a rem...

We describe a novel method for rapid and quantitative evaluation of the degree of radiation-induced apoptosis in the developing brain of medaka (Oryzias latipes). Embryos at stage 28 were irradiated with 1, 2, 3.5, and 5 Gy x-ray. Living embryos were stained with a vital dye, acridine orange (AO), for 1-2 h, and whole-mount brains were examined under an epifluorescence microscope. From 7 to 10 ...

Immunohistochemical study for epidermal growth factor (EGF) and epidermal growth factor receptor (EGFR) was performed on 222 specimens of gastric carcinoma. The authors placed each carcinoma into one of the following three groups: group 1, neither EGF nor EGFR was stained (123 cases); group 2, either EGF or EGFR was stained (64 cases); and group 3, both EGF and EGFR were stained (35 cases). Com...

Figure 9.1 Growth of proteomics. Using the search parameter “proteom*” the NCBI database was queried for citations. The number of citations in each year plotted against the year shows the tremendous increase in research and interest in the proteomics field. Figure 9.2 2-D protein gels of tumor vs. normal cells stained for glycoproteins and total proteins. Lysates from rat liver tumor cells (top...