PCR-restriction fragment length polymorphism (PCR-RFLP) and PCR-single-strand conformation polymorphism (PCR-SSCP) analyses were carried out on the 1.6-kb groEL gene from 41 strains of 10 different Salmonella serovars. Three HaeIII RFLP profiles were recognized, but no discrimination between the serovars could be achieved by this technique. However, PCR-SSCP analysis of the groEL genes of vario...

the growth hormone (gh) gene is a candidate for growth trait in farm animals and plays an important role in growth metabolism. growth hormone can contribute in growth metabolism when it's receptor (ghr) are on cells target. the single nucleotide polymorphism was occurred in gh and ghr genes and that associated with carcass traits in lori-bakhtiari sheep breed with using pcr-sscp. in this r...

The anisakid nematodes morphologically corresponding with Pseudoterranova decipiens sensu lato (s.l.) (Krabbe, 1878) from different seal or sea lion hosts and geographical origins, previously identified as Pseudoterranova krabbei, P. decipiens (s.s.), P. bulbosa, P. azarasi and P. cattani by multilocus enzyme electrophoresis, were characterized using a DNA approach. Also a population of P. deci...

Chromatin can be analysed by assaying its sensitivity to DNase I or other nucleases in purified nuclei. Usually, this is performed by Southern analysis of genomic DNA extracted from nuclease-treated nuclei, a methodology that requires many cells. Applying restriction fragment length polymorphisms (RFLPs), this methodology has been used for parental allele-specific chromatin studies on imprinted...

the growth hormone receptor (ghr) is a member of cytokine/hematopoietin family that mediates the biological actions of growth hormone (gh) on target tissues. therefore, the purpose of this study was to examine the association of tg-repeat polymorphisms in the 5’-flanking region of bovine ghr gene with milk production traits and somatic cell score (scs) in holstein cattle of iran. the part of 5’...

The aim of this study was to perform molecular and bioinformatics analysis of IGFBP2 gene promoter in association with some economic traits in indigenous Makuee (MS) and Lori-Bakhtiari (LB) breeds. DNA was extracted from blood samples of 120 MS and 200 LB and a 297 bp fragment from the upstream sequences of studied gene was amplified and genotyped by single-strand conformational polymo...

Forms of mutation never before described in the rpoB gene are reported for a sample of 20 rifampicin-resistant Mycobacterium avium Complex (MAC) strains isolated from AIDS patients in Thailand. All strains were analyzed by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and polymerase chain reaction-DNA sequencing (PCR-DNA sequencing). Sequence analysis of these str...

First-stage nematode larvae with a dorsal-spine (DSL) were detected in five of 1,565 fecal samples from gray wolves (Canis lupus) collected in British Columbia, Canada, between 2005 and 2008. Molecular techniques were used to identify the DSL because it was not possible to determine their species identity using morphologic characters. The DSL were identified as Parelaphostrongylus odocoilei bas...

A series of serial passages of Triticum mosaic virus (TriMV) was performed in winter wheat under greenhouse conditions to examine the dynamics of sequence variation in the TriMV population. The variation within the protein 1 (P1) and the coat protein (CP) of TriMV was assessed by single-strand conformational polymorphism (SSCP) assay, followed by nucleotide sequencing. Three lineages were estab...

objective: diacylglycerol acyltransferase 1 is a microsomal enzyme that catalyzes the final step of triglyceride synthesis. the objective of this project is to check out the polymorphism at the exon 8 region of dgat1 gene using pcr-sscp technique in iranian water buffaloes (bubalus bubalis). recent activities have shown that a significant association between lysine at amino acid position 232 wi...