OBJECTIVE Ischemic postconditioning protects the reperfused heart from infarction, and this protection is dependent on the occupancy of adenosine receptors. We further explored the role of adenosine receptors in this salvage. METHODS In situ rabbit hearts underwent 30 min of regional ischemia and 3 h of reperfusion, and postconditioning was effected with four cycles of 30-s reperfusion/30-s c...

The resolution of conventional surface-plasmon-resonance (SPR) imaging has been limited by the diffraction nature of light. A wide-field extended-resolution optical imaging technique, standing-wave SPR fluorescence (SW-SPRF) microscopy, has been developed. Based on evanescent SPR standing waves, SW-SPRF provides lateral resolution approaching 100 nm and offers the advantages of significant sign...

Simultaneous determination of wild-type and total p53 proteins (wild-type and mutant combined) present in cancer cell lysates has been performed with a dual-channel surface plasmon resonance (SPR) instrument. To achieve specificity, each channel of the SPR chip was modified with a consensus double-stranded (ds-) DNA and a monoclonal antibody. The high affinity of the consensus ds-DNA to the wil...

In this paper, we describe the detection of bacterial cell-extracted 16S ribosomal RNA (rRNA) using an emerging technology, surface plasmon resonance (SPR) imaging of DNA arrays. Surface plasmon resonance enables detection of molecular interactions on surfaces in response to changes in the index of refraction, therefore eliminating the need for a fluorescent or radioactive label. A variation of...

In previous studies, we have shown that loss of spinal neurons that possess the substance P receptor (SPR) attenuated pain and hyperalgesia produced by capsaicin, inflammation, and nerve injury. To determine the role of SPR-expressing neurons in modulating pain and hyperalgesia, responses of superficial and deep lumbar spinal dorsal horn neurons evoked by mechanical and heat stimuli and by caps...

Surface plasmon resonance (SPR) chips are widely used to measure association and dissociation rates for the binding kinetics between two species of chemicals, e.g., cell receptors and ligands. It is commonly assumed that ligands are spatially well mixed in the SPR region, and hence a mean-field rate equation description is appropriate. This approximation however ignores the spatial fluctuations...

Dietary fiber (DF) has attracted increasing interest from nutritionists. The yield of DF by traditional extraction methods, however, is very low. This paper aims to increase the yield of DF by solid state fermentation using the edible and medicinal fungus Schizophyllum commune. Sweet potato residue (SPR) was selected as raw material for producing DF. Results showed that SPR was a good feedstock...

The present study investigates the optical characteristics and the spectral and angular responses of a Kretschmann surface plasmon resonance (SPR) sensor configuration that is widely used in biological and chemical sensing applications. In order to examine the influence of wave interference and optical properties of thin films on angular variation of reflectance at different incident angles, th...

Due to their extreme sensitivity to refractive index changes, surface plasmon resonance (SPR) sensors have long been established as extremely valuable tools for biosensing. In the past few years researchers have begun investigating various other metallic nanostructures as candidates for localized SPR (LSPR) sensing. Although LSPR is not nearly as sensitive to bulk refractive index changes as st...