Culture of a mixture of bone marrow cells with and without T6 chromosome marker in diffusion chambers in mice yielded colonies (CFU-DG) containing cells of a single karyotype, suggesting clonality. Injection of individual CFU-DG colonies into lethally irradiated mice resulted in increased spleen colony formation on day 12 (CFU-S). The possibility of endogenous origin was excluded by demonstrati...

Hematopoietic efficacy in vivoof multiple injections of purified murine I -cell and recombinant human macrophage colony-stimulating factors (M-CSF; specific activity, >2 x IO7 units/mg) was assessed in mice. Injections i.v. of sterile saline or 20,000 units of M-CSF were adminis tered once (at 0 h), twice (at 0 and 12 h), or three times (at 0, 12, and 24 h) to C57BL/6 x DBA/2 F, mice. Numbers a...

Hematopoietic efficacy in vivo of multiple injections of purified murine L-cell and recombinant human macrophage colony-stimulating factors (M-CSF; specific activity, greater than 2 x 10(7) units/mg) was assessed in mice. Injections i.v. of sterile saline or 20,000 units of M-CSF were administered once (at 0 h), twice (at 0 and 12 h), or three times (at 0, 12, and 24 h) to C57BL/6 x DBA/2 F1 mi...

OBJECTIVE To develop an aerosol exposure method for induction of brucellosis in rhesus macaques (Macaca mulatta). ANIMALS 10 adult rhesus macaques. PROCEDURE 8 rhesus macaques were challenge exposed with 10(2) to 10(5) colony-forming units of Brucella melitensis 16M by use of an aerosol-exposure technique, and 2 served as control animals. All macaques were euthanatized 63 days after challen...

Lethally irradiated mice received a transfusion of normal bone marrow cells from the same strain mice. The transfused colony-forming cells (stem cells) were settled in the spleen of the recipient and proliferated in it into erythroblast colonies. Some of these mice were given chloramphenicol or thiamphenicol for 7 days after marrow cell transfusion. Annulate lamellae were frequently observed ex...

Hematopoietic reconstitution of ablated recipients requires that intravenously (IV) transplanted stem and progenitor cells "home" to organs that support their proliferation and differentiation. To examine the possible relationship between homing properties and subsequent engraftment potential, murine bone marrow (BM) cells were labeled with fluorescent PKH26 dye and injected into lethally irrad...

BACKGROUND AND OBJECTIVES Recombinant human granulocyte colony-stimulating factor (rhG-CSF) is widely used to mobilize peripheral blood stem cells (PBSC) for autologous or allogeneic transplants. Such treatment may cause spleen enlargement; exceptionally, spontaneous spleen rupture has been reported. We investigated changes in spleen size during stem cell mobilization. DESIGN AND METHODS We e...