The genes encoding the five subunits of the F1 portion of the ATPases from both spinach chloroplasts and the cyanobacterium Synechocystis sp. PCC 6803 were cloned into expression vectors and expressed in Escherichia coli. The recombinant subunits formed inclusion bodies within the cells. Each particular subunit was expressed in the respective unc mutant, each unable to grow on non-fermentable c...

The virus earlier reported from spinach (S. oleracea) from Yugoslavia under the tentative name of spinach latent virus has been shown — by symptomatology in test plants, properties in sap, transmission through seed and serological reactions — to be identical with the ilarvirus characterised under the same name in the Netherlands. After mechanical inoculation the Yugoslav isolate (SpLV/ /H) of s...

We have used protein cross-linking with the zero-length cross-linker 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide, and radiolytic footprinting coupled with high-resolution tandem mass spectrometry, to examine the structure of higher-plant PsbO when it is bound to Photosystem II. Twenty intramolecular cross-linked residue pairs were identified. On the basis of this cross-linking data, spinach ...

Fluorogenic RNAs that are based on the complex formed by 3,5-difluoro-4-hydroxybenzylidene imidazolinone (DFHBI) derivatives and the RNA aptamer named Spinach were used to engineer a new generation of in vitro and in vivo sensors for bioanalytics. With the resolved crystal structure of the RNA/small molecule complex, the engineering map becomes available, but comprehensive information regarding...

Abstract—There was a scenario present day that drying of fresh fruits and vegetables by indirect solar drying by using mechanical device; hence, an effort was made to develop a small scale solar tunnel dryer (STD). Drying of spinach is carried out to analyze the performance of the dryer and to study its drying characteristics. To evaluate the performance of dryer the independent variables were ...

The dry deposition to several leafy vegetables (endive, head lettuce, red oak leaf lettuce, spinach) was studied. Particles with attached Cs were used for testing the particulate deposition, gaseous elemental I was the tracer for gas deposition. The particle deposition was the highest for rough and structured leafs as red oak leaf lettuce has, but as well for spinach with many open stomata. The...