REČKOVá, Z., MACHATKOVá, M., RyBář, R., MáCHAL, L.: Monitoring of chromatin integrity changes in the population of motile bovine sperm capacitated in vitro. Acta univ. agric. et silvic. Mendel. Brun., 2007, LV, No. 2, pp. 65–70 The objective of our study was to standardize a method for chromatin integrity assessment in a separated population of bovine sperm and monitor the changes occurring dur...

Objective: To examine the qualitative and quantitative characteristics of frozen-thawed spermatozoa recovered through the SpermPrep (ZBL, Inc., Lexington, KY) filtration method or the swim-up technique for the purpclse of intrauterine insemination (IUI) or other techniques for assisted reproduction. Design, Setting, Patients: Thirty pairs of frozen specimens purchased from three commercial seme...

The aim of this study was to develop and to test the standardized aseptic technology of permeable cryoprotectant-free vitrification of human spermatozoa in capillaries (for intracytoplasmic sperm injection [ICSI] or in vitro fertilization [IVF]). To test the effect of vitrification on basic sperm parameters, each of 68 swim-up-prepared ejaculates from oligo-astheno-terato-zoospermic patien...

Caput epididymal wild-type spermatozoa and cauda epididymal spermatozoa from mice null for the adenylyl cyclase Adcy10 gene are immotile unless stimulated by a membrane-permeant cyclic AMP analogue. Both types of spermatozoa exhibit flagellar angulation where the head folds back under these conditions. As sperm proteins undergo oxidation of sulfhydryl groups and the flagellum becomes more stabl...

The purposes of this study were to examine the relationship between male artificial insemination (AI) fertility and sperm acrosomal conditions assessed by new and conventional staining techniques and to identify possible reproductive dysfunctions causing low conception rates in AI using frozen-thawed spermatozoa with poor acrosomal conditions in Japanese Black bulls. We investigated individual ...

Sperm morphology was evaluated (using Blom classification) in 44 ejaculates of 11 bulls and 44 ejaculates of 11 boars. Significant differences in sperm morphology were found between bulls and boars. In addition, the correlations between frequency of morphological changes and morphometrical traits of boar spermatozoa were demonstrated. More morphological anomalies of spermatozoa were recorded in...

We demonstrate a pinched flow tangential filtration method to sort spermatozoa from larger particles with a spermatozoa collection efficiency of 94±2% and a separation efficiency of 100%. In conventional pinched flow fractionation (PFF), an observed tumbling-like rotation of spermatozoa complicates their separation from other particles. The new design circumvents this effect.

BACKGROUND Sperm protein 17 (Sp17) is a three-domain protein that contains: 1) a highly conserved N-terminal domain that is 45% identical to the human type II alpha regulatory subunit (RII alpha) of protein kinase A (PKA); 2) a central sulphated carbohydrate-binding domain; and 3) a C-terminal Ca++/calmodulin (CaM) binding domain. Although Sp17 was originally discovered and characterized in spe...

Freeze-dried mouse spermatozoa can be used for normal embryonic development after injection into oocytes, thus indicating that freeze-drying is a useful method for the storage and transportation of genetic materials from animals. We recently reported that storage of freeze-dried mouse spermatozoa requires maintenance at temperatures lower than -80 C for long-term preservation and a pressure of ...

background: when male animals die, spermatozoa within the body of animal will be degenerated. because of unique chromatin structure of sperm, maybe this degeneration is different from other cells. however there is not any research which considered directly the integrity of sperm dna by keeping the cadaver in refrigerator. objective: the aim of this study was to assess viability, total motility ...