OBJECTIVE Testicular cell transplantation has been widely used to investigate the restoration of fertility in rodent models. In this research we apply transplantation as a treatment method in the cryptorchid model and compare this method with orchidopexy, which is the routine treatment for this problem. We studied the controversial effects of treatment on the number of germ cells and other morp...

The effects of 3 Gy local X-irradiation on the adult rat testis were studied together with exact determination of the radiation dose distribution in the testis. Seminiferous tubule segments were isolated 8-66 hours postirradiation (p.i.), squashed between a microscope slide and a coverslip, and the exact stage of the seminiferous epithelial cycle was identified under a phase-contrast microscope...

background: date palm pollen (dpp) and astragalus genus are used in some countries for the treatment of infertility. objective: this study was designed to investigate effects of dpp and astragalus ovinus (a.ovinus) on fertility in healthy adult male rats. materials and methods: thirty-six rats were divided into six groups (n=6) including control and five treatment groups. dpp (120, 240 and 360 ...

objective(s) urtica dioica l. has been known as a medicinal plant in the world. this study was conducted to determine the effects of the hydroalcoholic extract of urtica dioica leaves on seminiferous tubules of diabetic rats. materials and methods animals were allocated to control, diabetic and protective groups. treated animals received extract of u. dioica (100 mg/ kg/ day) ip for the first 5...

STUDY QUESTION Can human spermatogonia be detected in long-term primary testicular cell cultures using validated, germ cell-specific markers of spermatogonia? SUMMARY ANSWER Germ cell-specific markers of spermatogonia/spermatogonial stem cells (SSCs) are detected in early (1-2 weeks) but not late (> 6 weeks) primary testicular cell cultures; somatic cell markers are detected in late primary t...

Objective(s):Leptin is a novel and interesting hormone for anyone trying to lose weight, but its effects on male gonad structure in longitudinal study is unknown. The present study was designed to explore morphometrical changes of mouse testicular tissue after long-term administration of leptin. Materials and Methods:Thirty healthy mature male mice were randomly assigned to either control (n=15...

BACKGROUND Spermatogonia are highly tolerant to reactive oxygen species (ROS) attack while advanced-stage germ cells such as spermatozoa are much more susceptible, but the precise reason for this variation in ROS tolerance remains unknown. METHODOLOGY/PRINCIPAL FINDINGS Using the Japanese eel testicular culture system that enables a complete spermatogenesis in vitro, we report that advanced-s...

Spermatogenesis is a classic model of cycling cell lineages that depend on a balance between stem cell self-renewal for continuity and the formation of progenitors as the initial step in the production of differentiated cells. The mechanisms that guide the continuum of spermatogonial stem cell (SSC) to progenitor spermatogonial transition and precise identifiers of subtypes in the process are u...