Reactive oxygen species (ROS) generation, induced by the cryopreservation process, can be responsible for mammalian sperm damage. Curcumin is known as an effective antioxidant against oxidative stress. The aim of this study was to evaluate the effects of curcumin on sperm count, motility and viability, semen total antioxidant capacity and DNA integrity of rat spermatozoa during semen freeze-tha...

Problem statement: Boar sperm cells are sensitive to the freezing process, which compromises viability of frozen-thawed sperm. In a constant search for minimizing or suppressing sperm cell damage caused by the temperature and osmolarity changes during the freezing process, crioprotective and antioxidant substances have been added to the freezing media, such as coconut water, in order to increas...

Background: Obesity is a medical condition in which excess body fat has accumulated to the extent that it may have an adverse effect on health, leading to reduced life expectancy and/or increased health problems such as infertility and sub fertility. Lipid peroxidation contributes to the damage of the sperm plasma membrane and result to infertility. In this study effects of Antioxidants (E, C, ...

Background: Mammalian sperm are not ready to fertilize oocyte immediately after ejaculation. Sperm achieve this ability after undergoing the processes of capacitation and hyperactivation. Hyperactivation refers to a change in the motility pattern of the sperm. Pharmacological reagents and physical-mechanical forces have been used in sperm from a variety of species. Hydrostatic pressure is a cru...

Background: The importance of polyunsaturated fatty acids (PUFAs) on chilled sperm quality is under investigation. Addition of fatty acids to the diet can influence sperm quality and male fertilizing capacity. The aim of this study was to investigate if the addition of n-3 PUFAs improves quality of bovine chilled sperm. Materials and Methods: In order to do experiment, 5 proven bulls were rando...

BACKGROUND The hypo-osmotic swelling (HOS) test evaluates the ability of the functional sperm plasma membrane to stretch following cell swelling when exposed to hypo-osmotic solutions. Sperm samples with low HOS scores show low fertilization and pregnancy rates during assisted reproductive techniques, though data are controversial. The aim of this study was to compare the results of the HOS tes...

Background Low levels of reactive oxygen species (ROS) and calcium are necessary for sperm function. NADPH oxidase 5 (NOX5) is a membrane enzyme which produces ROS. This enzyme is dependent on calcium for its activity. We investigated the importance of NOX5 and an important calcium channel (CatSper) on sperm function. Methods This laboratory in-vitro study was done in Shiraz, Iran, 2016. Norm...

Spermatozoa sexing requires a diluent that can protect and provide an optimal environment to maintain the quality of spermatozoa. This study targets determine effectiveness adding sucrose tris-egg yolk on sperm swamp buffaloes produced by with egg albumin method. Semen was collected once week for 12 consecutive weeks artificial vagina from three male at Indonesian Center Animal Research Develop...

The present study determined the effect of different types of sugars (lactose, fructose, glucose and sorbitol) used in egg yolk-based extender on the post-thawed boar semen quality. Twenty-two ejaculates from 6 fertility-proven Yorkshire boars were cryopreserved by liquid nitrogen vapor method. Sperm motility, viability, acrosome integrity and intact functional plasma membrane were determined a...