Objective(s): Sperm cryopreservation plays an undeniable role in assisted reproductive technology. However, this process significantly reduces the motility, viability, morphology and nuclear integrity of sperm. Reasons of these changes were oxidative stress and apoptosis. The aim of this study was to evaluate the influence of vitamin D on the survival and integrity of ...

Studies showed that assisted reproductive technology changed the sex ratio at birth, but the effect of semen processing (swim-up technique and density-gradient separation) on enriching either Xor Y-bearing sperm is always controversial. We recruited 30 healthy males and their semen samples were obtained by masturbation after sexual abstinence for 2-7 days. Each sample was divided into 3 aliquot...

This investigation was undertaken as an efficient method for isolating sperm DNA from a mixed fluid sample which contains vaginal epithelial cells in a greater amount. The modified method of the two-step differential extraction procedure was found to be suitable for separating sperm DNA and vaginal epithelial cell DNA from the mixed stains. As the first step of digestion, vaginal epithelial cel...

OBJECTIVE To determine the influence of the concentration of oval spermatozoa according to the strict morphology criterion in men with normal sperm concentration following the World Health Organization criteria on the results of classic IVF. MATERIALS AND METHODS Based on review of patient charts, this study included infertile couples presenting with female causes for infertility or unexplain...

The accurate measurement of semen fertilizing potential is of great importance in determining the acceptability of processed semen for breeding purposes. A good sperm preparation technique results in a sample with high viability and motility and also takes into account other parameters such as the capacitation and apoptotic state which could compromise the ability to fertilize an oocyte. In thi...

OBJECTIVE To compare the percentage of sperm tail membrane swelling under hypo-osmotic conditions between sperm treated with pentoxifylline and 2-deoxyadenosine. DESIGN Experimental in vitro study. MATERIAL AND METHOD Thirty normal semen samples from male partners of infertile couples were collected. After sperm preparation by two-layer Percoll gradient method, each sperm sample was divided...

Background: It is shown that hypertension may affect androgens. The role of blood pressure on sperm parameters is matter of debate. The main goal was to evaluate the probable association between systolic and diastolic blood pressure and sperm parameters. Materials and Methods: Semen Sample of men (aged: 27- 45 years old) from 40 infertile cases who attended at Yazd research and clinical center ...

The effect of seminal plasma, as well as some seminal plasma fractions, on the heterogeneity and viability of frozen-thawed ram spermatozoa was studied. Fresh and frozen sperm samples were simultaneously assessed by a routine semen-quality assay and centrifugal countercurrent distribution (CCCD) in an aqueous two-phase system analysis. All samples used for this study were obtained from Salz ram...

Background: Detection of sperm parameters based on conventional methods, immediately after thawing, have been revealed sperm capacity to in vivo and in vitro fertilization. But this evaluation is not fully reliable because of long journey of sperm during female genital tract to reach the oocyte and fertilize it. Therefore, analyzing postthawed sperm parameters after incubation is more beneficia...