During spermatogenesis, sperm chromatin undergoes structural changes and results in a high condensation. This nuclear compaction would be useful as a predictor of sperm fertilization capacity and pregnancy outcome. We purpose to evaluate firstly the relationship among chromatin maturity assessed by aniline blue staining (AB) and the semen parameters in infertile men. Secondly, we analyzed wheth...

background: standardization of the semen analysis may improve reproducibility. we assessed variability between laboratories in semen analyses and evaluated whether a transformation using z scores and regression statistics was able to reduce this variability. materials and methods: we performed a retrospective cohort study. we calculated between-laboratory coefficients of variation (cvb) for spe...

background: chromomycin a3 (cma3) staining has been used to assess protamine deficiency. the aim of this study was to determine credibility of cma3 along with semen parameters for assessment of fertility potential. materials and methods: semen analysis and cma3 staining were carried out on 234 fertile and 178 subfertile individuals. semen analysis was assessed according to who criteria. protami...

The aim of the study was to conduct a structured review of the literature published on the use of normal sperm morphology, as an indicator of male fertility potential in the in-vitro fertilization (IVF) situation, and to establish the universal predictive value of this semen parameter. Published literature in which normal sperm morphology was used to predict fertilization and pregnancy, during ...

background: high level of semen reactive oxygen species is considered as an important factor in male infertility. sesame has antioxidant properties, which could be effective on improvement of semen parameters. this study was designed to determine the effects of sesame on sperm quality.   materials and methods: twenty‑five infertile men entered this clinical trial. they were treated with a 3â€...

Currently, the delicate process of selecting sperm cells to be used for in vitro fertilization (IVF) is still based on the subjective, qualitative analysis of experienced clinicians using non-quantitative optical microscopy techniques. In this work, a method was developed for the automated analysis of sperm cells based on the quantitative phase maps acquired through use of interferometric phase...

In this study, the comparative effects of half, normal and double clinical doses of artesunate; artesunate/sulfadoxine/pyrimethamine; artesunate/amodiaquine and their combinants [sulfadoxine/pyrimethamine and amodiaquine] on the semen parameters namely total sperm count, motility, morphology, debris and premature/primordial cell count of the male guinea-pig were investigated. The agents caused ...

Background & objectives: Environmental pollutants have detrimental effects on the reproductive function of human being and of animals. This study was aimed at evaluating the effects of tire debris, a fraction of particulate matter derived from tire wear, on motility and morphology of human spermatozoa. Methods: spermatozoa from ten healthy men were incubated for 4 hours with 10, 50 and 75 μg/ml...

Despite controversy regarding the clinical value of semen analysis, male fertility investigation still relies on a standardized analysis of the semen parameters. This is especially true for infertility clinics in both developing and developed countries. Other optional tests or sophisticated technologies have not been widely applied. The current review addresses important changes in the analysis...

BACKGROUND Ubiquitin, a house-keeping protein that marks other proteins for proteasomal degradation, tags defective sperm during epididymal passage. To establish ubiquitin as a biomarker of human infertility, the present study examines the relationships between sperm ubiquitin content and clinical semen parameters among men from an infertility clinic population with varied aetiologies. METHOD...