Background: Aberrant promoter methylation is a major mechanism for silencing tumor suppressor genes in cancer. Detection of hypermethylation is used as a molecular marker for early cancer diagnosis, as a prognostic index, or to define therapeutic targets for reversion of aberrant methylation. We report on a novel signal generation technology for real-time PCR to detect gene promoter methylation...

A Chlamydophila psittaci species-specific real-time PCR targeting the rDNA ribosomal spacer was developed as well as a genotype-specific real-time PCR targeting the Cp. psittaci outer membrane protein A (ompA) gene. The SYBR Green-based species-specific real-time PCR detected Cp. psittaci genotypes A to F, and the recently discovered E/B genotype. The genotype-specific real-time PCR could easil...

Hand and face gestures are modeled using an appearance-based approach in which patterns are represented as a vector of similarity scores to a set of view models deened in space and time. These view models are learned from examples using unsupervised clustering techniques. A supervised learning paradigm is used to interpolate view scores into a task-dependent coordinate system appropriate for re...

Abstract. This paper presents a real-time property specific reduction approach for Time Petri Net (TPN). It divides TPN models into sub-nets of smaller size, and constructs an abstraction of reducible ones, which exhibits the same property specific behavior, but has less transitions and states. This directly reduces the amount of computation needed to generate the whole state space. This method...

Antibody-fluorophore conjugates have high potential for the specific fluorescence detection of target cancer cells in vitro and in vivo. However, the antibody-fluorophore conjugates described to date are inappropriate for real-time imaging of target cells because removal of unbound antibody is required to reduce background fluorescence before quantifiable analysis by microscopy. In addition, cl...

The contributions of this paper are twofold. First, we present a new type of dictionary that is intended as a search assistance in topic-specific Web searching. The method to construct the dictionary is a general method that can be applied to any reasonable topic. The first implementation deals with climate change. The dictionary has the following new features compared to standard dictionaries ...

The use of UML for developing real-time systems is rapidly gaining attention from the industry. The future release of a response to the OMG RFP for a “UML profile for performance, scheduling and time” [1] will probably boost the acceptance of UML by the real-time development community. The team formed inside OMG’s Real-Time Analysis and Design working group, preparing the response to the above ...

Information on weed distribution within the field is necessary to implement spatially variable herbicide application. Since hand labor is costly, an automated weed control system could be feasible. This paper deals with the development of an algorithm for real time specific weed recognition system based on Histogram Analysis of an image that is used for the weed classification. This algorithm i...