quick identification of vibrio cholerae in epidemics is   important, on the other hand; conventional methods are time-consuming and   costly. the aim of this study was to develop a rapid, inexpensive and high   sensitivity method for quick identification of vibrio cholerae . for   this purpose we designed a pcr detection based on magnetic nanoparticles for   identification of bacterial dna by p...

A highly sensitive one-step multiplex RT-PCR (OMRT-PCR) system with an internal plant gene control was developed to simultaneously detect single and mixed RNA DNA viral pathogens affecting watermelon squash in the Southeastern United States. Current methods rely on a singleplex detection for several viruses which is time-consuming large-scale field monitoring. The gene-specific primers one viru...

mycoplasma synoviae (ms) is an important avian pathogen that can cause both respiratory disease and joint inflammation synovitis in poultry, inducing economic losses to the iranian chicken industry especially breeder farms. the aim of this study was to use the ms specific pcr and culture methods in order to detect of m. synoviae from breeder farms where located in tehran province. a total of 47...

Allele-specific polymerase chain reaction (AS-PCR) is a rapid and cost-effective single nucleotide polymorphism (SNP) genotyping method compared to multiplex or real-time PCR. The SNPs occurring in mitochondrial DNA (mtSNPs) the most abundant humans can be used human identification involving mass fatality incidents. Nevertheless, application of AS-PCR has yet widely applicable forensic investig...

although mycoplasma equigenitalium has been implicated in equine reproductive problems, its prevalence is largely unexplored due to the lack of specific diagnostic tests. to address this limitation, the authors developed and optimized species-specific primer pairs that target m. eguigenitalium rpob (rna polymerase b subunit) gene sequences. the specificity of the pcr assay developed in this stu...