results the prevalence’s of aa, ac and cc genotypes were 55.2%, 24.1% and 20.7% in cad patients and 51.6%, 40.9% and 7.5% in the control subjects, respectively. the frequencies of the c allele were significantly higher in cad patients compared with control groups (p < 0.05). logistic regression analysis revealed a significant association between the c allele and the risk of cad (or = 1.61, 95%c...

The purpose of this study was to determine the effects aerobic exercise on carotid femoral pulse wave velocity (cf-PWV), cell adhesion molecules (intracellular (ICAM-1), vascular (VCAM-1), endothelial selectin (E-selectin), and oxidized LDL in elderly women aged 70–85 years, identify effect correlation with stiffness. Forty participants were recruited divided into three groups; stiffness (VSG, ...

L-selectin belongs to the C-type lectin family of glycoproteins and is constitutively expressed on most leukocytes. L-selectin mediates leukocyte rolling in inflamed microvessels and high endothelial venules (HEV) via binding to specific carbohydrate structures on selectin ligands. Previous studies using sialidase treatment suggested a role of sialic acid residues in L-selectin-dependent rollin...

L-selectin is a leukocyte lectin that mediates leukocyte capture and rolling in the vasculature. The cytoplasmic domain of L-selectin has been shown to regulate leukocyte rolling. In this study, the regulatory mechanisms by which this domain controls L-selectin adhesiveness were investigated. We report that an L-selectin mutant generated by truncation of the COOH-terminal 11 residues of L-selec...

It is known that L-selectin binds to glycoconjugates containing the tetrasaccharide sialyl Lewis X in a Ca2+-dependent manner. In addition, a number of other acidic oligosaccharides (for example heparin or chondroitin sulphate) or glycolipids (for example sulphatides) bind to L-selectin independent of cations. In this paper we have established that L-selectin binds to charged phospholipids, suc...

A growing body of evidence suggests that L-selectin ligands presented on circulating tumor cells facilitate metastasis by binding L-selectin presented on leukocytes. Commonly used methods for detecting L-selectin ligands on tissues, e.g., immunostaining, are performed under static, no-flow conditions. However, such analysis does not assay for functional L-selectin ligands, specifically those li...

P-selectin (CD62P) is a member of the selectin family of adhesion molecules involved in the regulation of leukocyte traffic. P-selectin glycoprotein ligand-1 (PSGL-1) is a mucin-like molecule that is thought to be a primary ligand for P-selectin. The interaction of P-selectin with PSGL-1 results in leukocyte rolling and recruitment of leukocytes to sites of inflammation and tissue injury. Howev...

We used in vitro and in vivo approaches to examine whether tumor necrosis factor-alpha (TNF-alpha) and oncostatin M (OSM), cytokines that bind to distinct classes of receptors, differentially regulate expression of P- and E-selectin in murine and primate endothelial cells. In human umbilical vein endothelial cells, TNF-alpha rapidly increased mRNA for E-selectin but not P-selectin. OSM elicited...

We used in vitro and in vivo approaches to examine whether tumor necrosis factor-a (TNF-a) and oncostatin M (OSM), cytokines that bind to distinct classes of receptors, differentially regulate expression of Pand E-selectin in murine and primate endothelial cells. In human umbilical vein endothelial cells, TNF-a rapidly increased mRNA for E-selectin but not P-selectin. OSM elicited little or no ...

Leukocyte recruitment into inflammatory sites is initiated by a reversible transient adhesive contact with the endothelium called leukocyte rolling, which is thought to be mediated by the selectin family of adhesion molecules. Selectin-mediated rolling precedes inflammatory cell emigration, which is significantly impaired in both P- and L-selectin gene-deficient mice. We report here that approx...