Antisera were raised by immunizing rabbits with mouse testicular germ cells, and absorbed in vivo by injection into castrated male whole mice to obtain a specific antiserum which reacted with mouse germ cells. The expression of mouse testis-specific antigenic macromolecules was then studied immunochemically with the antiserum. Approximately 20 antigenic macromolecules with molecular weights ran...

Although mutations may represent attractive targets for immunotherapy, direct identification of mutated peptide ligands isolated from human leucocyte antigens (HLA) on the surface of native tumour tissue has so far not been successful. Using advanced mass spectrometry (MS) analysis, we survey the melanoma-associated immunopeptidome to a depth of 95,500 patient-presented peptides. We thereby dis...

Immunodiffusion analysis of intact, sonically treated, and sonically treated and heat-treated cell antigen preparations of isolates of races 0 and 1 of Xanthomonas cyamopsidis of guar, Cyamopsis tetragonoloba, indicated that these races differ from one another antigenically. The differentiating precipitin reactions are believed to have been brought about by specific heat-stable surface antigens...

We have studied 19 S107 heavy chain variable region gene (VH11)-encoded monoclonal antibodies from NZBWF1 mice. These studies show that a single VH gene can encode both antibodies to foreign antigens (anti-phosphorylcholine) and to self antigens (anti-double-stranded DNA) in the same animal. All of the anti-DNA antibodies contain many somatic mutations compared with the relevant germline genes....

Over the past decade, it has been demonstrated that cancer is immunogenic, and multiple tumor antigens have been identified in cancer patients. It is now possible to potentially harness the immune response elicited by cancer growth as a potential diagnostic tool. Humoral immunity, or the development of autoantibodies against tumor-associated proteins, may be used as a marker for cancer exposure...

It has been shown that phase variation of Sh. sonnei is accompanied by changes in morphology and antigenic structure. Two mutants of the Phase I organism (II(S) and II(R)), which were studied, elaborate somatic antigens which are immunologically identical. The purified lipocarbohydrate-protein antigens of the Phase I and II(S) microorganisms are chemically similar yet immunologically distinct a...

Natural and immune human antibodies reactive with heat-labile and heat-stable antigens of virulent Neisseria gonorrhoeae were studied by use of an indirect fluorescent-antibody (IFA) procedure. The immunoglobulin class of the reactive antibodies was identified by using fluorescein-conjugated antisera specific for human IgG, IgA, or IgM in the IFA procedure. The effects of heat and mercaptoethan...

We have isolated UV light-induced and spontaneous tumors along with nonmalignant cells and tissues from each host. CD8+ CTL clones generated to a number of highly immunogenic UV-induced tumors did not react with autologous normal fibroblasts nor with autologous second tumors. Using up to 25 independently induced tumors as targets, these CTL clones were found to be uniquely specific for the part...

The antigen binding specificity of an antibody molecule is determined by the amino acid sequences of the variable (V) 1 regions of the heavy (H) and light (L) chains. The antibody repertoire of an individual is large and complex. The studies of antibody polypeptides and genes in mice have suggested three sources of diversity: multiple germline V genes (1-4), rearrangement of three sets of H cha...

Antigens that are tumor specific yet retained by tumor cells despite tumor progression offer stable and specific targets for immunologic and possibly other therapeutic interventions. Therefore, we have studied two CD4(+) T cell-recognized tumor-specific antigens that were retained during evolution of two ultraviolet-light-induced murine cancers to more aggressive growth. The antigens are riboso...