Changes in microbial communities associated with nematode control were studied by comparing population numbers of fungi and bacteria in the soil and in internal root tissues (endorhiza) in non-amended and chitin-amended soils. Addition of chitin to soil at 1% (w/w) eliminated plant-parasitic nematodes in a first planting of cotton cv. ‘Rowden’ and significantly reduced Meloidogyne incognita inf...

Once released, manure-borne bacteria can enter runoff via interaction with the thin mixing layer near the soil surface. The objectives of this work were to document temporal changes in profile distributions of manure-borne Escherichia coli and enterococci in the near-surface soil layers after simulated rainfalls and to examine differences in survival of the two fecal indicator bacteria. Rainfal...

Plant growth can be affected by soil bacteria. In turn, plants are known to influence soil bacteria through rhizodeposits and changes in abiotic conditions. We aimed to quantify the phylotype richness and relative abundance of rhizosphere bacteria that are actually influenced in a plant species-specific manner and to determine the role of the disproportionately large diversity of low-abundance ...

Peptonized milk agar with Actidione proved to be superior to either Trypticase soy agar or soil extract agar for enumeration of soil bacteria.

water for agriculture is one of the most important factors in arid and semi-arid areas and municipal wastewater treatment is an important resource for this purpose. therefore, potential of transfer contaminations is a serious problem regarding use of treated wastewater for agriculture. due to the risk of transfer contaminations through the use of wastewater, the study of transfer microbes in so...

To understand the response of soil bacteria to the surrounding environment, it is necessary to examine the gene expression profiles of the bacteria in the soil. For this purpose, we developed a new method of extracting RNA from soil reproducibly. Using this new method, we extracted RNA from a field soil, which was sterilized and inoculated with Rhodococcus sp. strain RHA1, a biphenyl degrader i...

This study investigated the degradation of sulfonamide antibiotics (SAs) and microbial community changes in sludge-amended soil. In batch experiments, SA degradation was enhanced by addition of spent mushroom compost (SMC), SMC extract, and extract-containing microcapsule, with SMC showing higher SA degradation rate than the other additives in soil-sludge mixtures. In bioreactor experiments, th...

A detailed understanding of microbial ecology in different supraglacial habitats is important due to the unprecedented speed of glacier retreat. Differences in bacterial diversity and community structure between glacial snow and glacial soil on the Chongce Ice Cap were assessed using 454 pyrosequencing. Based on rarefaction curves, Chao1, ACE, and Shannon indices, we found that bacterial divers...

The complexities of the relationships between plant and soil microbial communities remain unresolved. We determined the associations between plant aboveground and belowground (root) distributions and the communities of soil fungi and bacteria found across a diverse tropical forest plot. Soil microbial community composition was correlated with the taxonomic and phylogenetic structure of the abov...