UNLABELLED We evaluated the interaction of [3H]1,25(OH)2D3 with skin fibroblasts cultured from normal subjects or from affected members of six kindreds with rickets and resistance to 1-alpha, 25(OH)2D [1,25(OH)2D]. We analyzed two aspects of the radioligand interaction; nuclear uptake with dispersed, intact cells at 37 degrees C and binding at 0 degrees C with soluble extract ("cytosol") prepar...

Extraction of rat liver cytosol with 10% charcoal at 4 degrees C inactivates specific glucocorticoid-binding capacity. The steroid-binding capacity of extracted cytosol can be restored by adding dithiothreitol or by incubating with boiled liver cytosol at 20 degrees C in the presence of 10 mM sodium molybdate. Two components of boiled cytosol are required for receptor activation: NADPH and an e...

A comprehensive study of the isomer-independent synthesis of TRIS ((HOCH(2))(3)CNH(2)) Mn-Anderson compounds from Na(2)MoO(4)·2H(2)O, via the corresponding octamolybdate species, is presented. Three octamolybdate salts of [Mo(8)O(26)](4-) in the β-isomer form, with tetramethylammonium (TMA), tetraethylammonium (TEA) and tetrapropylammonium (TPA) as the counter cation, were synthesised from the ...

Hydrogen is a selective inhibitor for nitrogen fixation, the effect in aerobes being one of competition with nitrogen (Wilson, 1951; Rosenblum and Wilson, 1950; Pratt and Frenkel, 1959). Also, carbon monoxide and nitrous oxide are selective fixation inhibitors (Hino, 1955) in Clostridium pasteurianum. The present work was undertaken to provide additional examples of selective inhibitors for nit...

Abstract Sodium molybdate (Na 2 MoO 4 ) has been synthesized and investigated as a heterogeneous solid catalyst for biodiesel from Camelina sativa seed oil. Transesterification reactions occurred under atmospheric conditions with relatively, low temperature short reaction time normal pressure. The prepared was characterised by means of SEM, TGA, UV, XRD FTIR. properties the were compared intern...

Active transport of exogenous sulfate into log phase cells of Porphyridium aerueineum followed Michaelis-Menten kinetics, and the apparent Km for sulfate transport is approximately 2.5 x 10(-6)m. Molybdate, also a group VI anion, is a competitive inhibitor of sulfate transport, and the inhibition is freely reversible. Once in the cell, molybdate depresses the rate of sulfate pool utilization by...

A sequential injection analysis system (SIA) is described which is suited for the fast determination of filterable molybdate reactive phosphate (FRP, 0.2mum) in coastal waters. It processes up to 270 samples per hour with a detection limit (3sigma) of 0.05muM and is used for surface mapping of phosphate in areas with steep concentration gradients like the Wadden Sea. The determination is based ...

Remarkable improvement in the polarization and charge transport at solid–solid electrode–electrolyte interface due to incorporation of a redox-active additive sodium molybdate (Na2MoO4) is demonstrated. The presence electrochemically active oxide center along with Na+ facilitates hopping-based ionic liquid leading higher conductivity twofold increase specific capacitance (0.009 0.018 F cm−2). T...